Ragupathy Subramanyam, Thirugnanasambandam Arunachalam, Vinayagam Varathan, Newmaster Steven G
Natural Health Products (NHP) Research Alliance, College of Biological Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Plants (Basel). 2024 Mar 14;13(6):841. doi: 10.3390/plants13060841.
(syn ) is considered 'true' cinnamon. However, it is reported that less expensive sources of cinnamon from (syn ), , and (toxic coumarin) may be used in the place of . We lack the quality assurance tools that are required to differentiate from other cinnamon species when verifying that the correct species is sourced from ingredient suppliers. The current research on cinnamon species authentication using DNA tools is limited to a few species and the use of high-quality DNA extracted from raw leaf materials. The cinnamon bark traded in the supply chain contains much less DNA and poorer-quality DNA than leaves. Our research advances DNA methods to authenticate cinnamon, as we utilized full-length chloroplast genomes via a genome skimming approach for and to facilitate the design of optimal mini DNA markers. Furthermore, we developed and validated the use of NMR fingerprints for several commercial cinnamon species, including the quantification of 16 molecules. NMR fingerprints provided additional data that were useful for quality assessment in cinnamon extract powders and product consistency. Both the new mini DNA markers and NMR fingerprints were tested on commercial cinnamon products.
（桂皮）被认为是“真正的”肉桂。然而，据报道，来自（锡兰肉桂）、（中国肉桂）和（含香豆素，有毒）的价格较低的肉桂来源可能会被用来替代（桂皮）。在核实从原料供应商采购的是正确品种时，我们缺乏区分（桂皮）与其他肉桂品种所需的质量保证工具。目前利用DNA工具对肉桂品种进行鉴定的研究仅限于少数几个品种，且使用的是从生叶材料中提取的高质量DNA。供应链中交易的肉桂树皮所含的DNA比树叶少得多，质量也较差。我们的研究改进了用于鉴定肉桂的DNA方法，因为我们通过基因组鸟枪法利用全长叶绿体基因组对（桂皮）和（中国肉桂）进行分析，以促进最佳微型DNA标记的设计。此外，我们开发并验证了几种商业肉桂品种的核磁共振指纹图谱的使用，包括对16种分子的定量分析。核磁共振指纹图谱提供了额外的数据，有助于对肉桂提取物粉末进行质量评估和保证产品一致性。新的微型DNA标记和核磁共振指纹图谱都在商业肉桂产品上进行了测试。
