Laboratory of Natural Products and Phytochemistry, Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama, 350-0295, Japan.
Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 210-9501, Japan.
J Nat Med. 2022 Jan;76(1):87-93. doi: 10.1007/s11418-021-01554-6. Epub 2021 Aug 6.
Cinnamon bark is an important spice worldwide. In this study, the chemical diversity of various commercially available cinnamon barks that differed in their production areas and utility applications (culinary spice or medicines) were investigated by the use of H NMR metabolomics. Our results indicated that principle component analysis (PCA) and hierarchical cluster analysis (HCA) of the H NMR spectra of the cinnamon bark methanolic extracts including the deduction of their species by nucleotide sequence analysis enabled differentiation of the cinnamon barks according to their species, production areas and utility applications. The constituents of Vietnam cinnamon were found to differ significantly from the other samples investigated based on PCA score plots and HCA constellation dendrograms. Coumarin was found to be a key compound for the discrimination of Vietnamese cinnamon by multivariate analysis of the H NMR spectral data and direct comparison of the H NMR spectra. In addition, coumarin was quantified using quantitative NMR methods. As a result, coumarin was contained in Vietnamese cinnamon at a higher level compared to other cinnamons. This study indicated that H NMR metabolomics could deduce spices, utility, and producing area of commercially available cinnamon barks. Furthermore, combining quantitative H NMR methods with H NMR metabolomics enable quantification of coumarin in cinnamon bark on a single measurement.
肉桂皮是一种在世界范围内都很重要的香料。在这项研究中，我们使用 H NMR 代谢组学研究了不同产地和用途（烹饪香料或药物）的各种市售肉桂皮的化学多样性。我们的结果表明，肉桂皮甲醇提取物的 H NMR 光谱的主成分分析（PCA）和层次聚类分析（HCA），包括通过核苷酸序列分析推断其种类，可以根据种类、产地和用途对肉桂皮进行区分。基于 PCA 得分图和 HCA 树状图，越南肉桂的成分与其他调查样本有显著差异。多元分析 H NMR 光谱数据和直接比较 H NMR 光谱表明，香豆素是区分越南肉桂的关键化合物。此外，使用定量 NMR 方法对香豆素进行了定量。结果表明，与其他肉桂相比，越南肉桂中香豆素的含量更高。这项研究表明，H NMR 代谢组学可以推断市售肉桂皮的香料、用途和产地。此外，将定量 NMR 方法与 H NMR 代谢组学相结合，可以在单次测量中定量肉桂皮中的香豆素。
