Hyink Otto, Dellow Ross A, Olsen Michael J, Caradoc-Davies Katherine M B, Drake Kylie, Herniou Elisabeth A, Cory Jennifer S, O'Reilly David R, Ward Vernon K
Department of Microbiology, Otago School of Medical Sciences, University of Otago, PO Box 56, Dunedin, New Zealand1.
Ecology and Biocontrol Group, Centre for Ecology and Hydrology, Mansfield Road, Oxford OX1 3SR, UK3.
J Gen Virol. 2002 Apr;83(Pt 4):957-971. doi: 10.1099/0022-1317-83-4-957.
The nucleotide sequence of the Epiphyas postvittana nucleopolyhedrovirus (EppoMNPV) genome has been determined and analysed. The circular dsDNA genome contains 118584 bp, making it the smallest group I NPV sequenced to date. The genome has a G+C content of 40.7% and encodes 136 predicted open reading frames (ORFs), five homologous repeat regions and one unique repeat region. Of the genome, 92.9% encodes predicted ORFs and 2.2% is in repeat regions; the remaining 4.9% of the genome comprises nonrepeat intergenic regions. EppoMNPV encodes homologues of 126 Orgyia pseudotsugata MNPV (OpMNPV) ORFs and 120 Autographa californica MNPV ORFs, with average identities of 64.7 and 53.5%, respectively. Between the four sequenced group I NPVs, 117 ORFs are conserved, whereas 86 ORFs are conserved between all fully sequenced NPVs. A total of 62 ORFs is present in all baculoviruses sequenced to date, with EppoMNPV lacking a homologue of the superoxide dismutase (sod) gene, which has been found in all other fully sequenced baculoviruses. Whole genome phylogenetic analyses of the ten fully sequenced baculoviruses using the sequences of the 62 shared genes, gene content and gene order data sets confirmed that EppoMNPV clusters tightly with OpMNPV in the group I NPVs. The main variation between EppoMNPV and OpMNPV occurs where extra clusters of genes are present in OpMNPV, with sod occurring in one such cluster. EppoMNPV encodes one truncated baculovirus repeated ORF (bro) gene. The only repeated ORFs are the four iap genes. Eight, randomly distributed, unique ORFs were identified on EppoMNPV, none of which show any significant homology to genes in GenBank.
苹果蠹蛾核多角体病毒（EppoMNPV）基因组的核苷酸序列已被测定和分析。环状双链DNA基因组包含118584碱基对，使其成为迄今为止测序的最小的I组NPV。该基因组的G+C含量为40.7%，编码136个预测的开放阅读框（ORF）、五个同源重复区域和一个独特重复区域。基因组中，92.9%编码预测的ORF，2.2%位于重复区域；基因组其余4.9%由非重复基因间隔区组成。EppoMNPV编码126个云杉芽卷蛾核多角体病毒（OpMNPV）ORF和120个苜蓿银纹夜蛾核多角体病毒ORF的同源物，平均同一性分别为64.7%和53.5%。在四个已测序的I组NPV之间，117个ORF是保守的，而在所有完全测序的NPV之间有86个ORF是保守的。在迄今为止测序的所有杆状病毒中共有62个ORF，EppoMNPV缺乏超氧化物歧化酶（sod）基因的同源物，而在所有其他完全测序的杆状病毒中都发现了该基因。使用62个共享基因的序列、基因含量和基因顺序数据集对十个完全测序的杆状病毒进行全基因组系统发育分析，证实EppoMNPV在I组NPV中与OpMNPV紧密聚类。EppoMNPV和OpMNPV之间的主要差异发生在OpMNPV存在额外基因簇的地方，sod基因就位于其中一个这样的簇中。EppoMNPV编码一个截短的杆状病毒重复ORF（bro）基因。唯一重复的ORF是四个iap基因。在EppoMNPV上鉴定出八个随机分布的独特ORF，其中没有一个与GenBank中的基因显示出任何显著同源性。
