Prete Chiara Del, Nocera Francesca Paola, Piegari Giuseppe, Palumbo Veronica, Martino Luisa De, Cocchia Natascia, Paciello Orlando, Montano Chiara, Pasolini Maria Pia
Reproduction Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Diagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Vet World. 2024 Feb;17(2):398-406. doi: 10.14202/vetworld.2024.398-406. Epub 2024 Feb 16.
A combined microbial and cytological examination of uterine samples is the main diagnostic method for endometritis in mares. This study aimed to describe a procedure for using the same uterine cytobrush (CB) for both bacteriological and cytological evaluation.
The procedure consists of rolling the CB onto a sterilized glass slide immediately after collection and before the transfer into a sterile saline solution. In Experiment 1, a comparison between bacteriological results of the cotton swab (CS) and CB or pellet was made in 10 mares; in Experiment 2, bacteriological and cytological results were compared between different processing methods of CB in 28 mares; in other 6 mares, a CB was processed for cytology only, to investigate the reasons for the low cellularity of the pellet.
The agreement between culture results from the CB and CS was evaluated, and a comparison between the cytological data obtained by different processing methods of CB was performed. The perfect agreement between the CB and CS microbiological results was found. The described procedure enables useful diagnostic smears for cytology. Moreover, the seeding of both the tip of CB and the saline solution used for the transport produced accurate bacteriological results.
The protocol described in this study for the use of CB for both cytological and bacteriological analysis could be used for the diagnosis of endometritis. To maximize diagnostic sample quality, cytology slides must be prepared with meticulous care in the field to preserve cellular integrity and minimize artifacts.
对母马子宫样本进行微生物学和细胞学联合检查是子宫内膜炎的主要诊断方法。本研究旨在描述一种使用同一子宫细胞刷（CB）进行细菌学和细胞学评估的方法。
该方法包括在采集后、转移至无菌盐溶液前，立即将CB在无菌载玻片上滚动。在实验1中，对10匹母马的棉拭子（CS）、CB或沉淀物的细菌学结果进行比较；在实验2中，对28匹母马CB的不同处理方法的细菌学和细胞学结果进行比较；在另外6匹母马中，仅对CB进行细胞学处理，以研究沉淀物细胞数量少的原因。
评估了CB和CS培养结果之间的一致性，并对CB不同处理方法获得的细胞学数据进行了比较。发现CB和CS微生物学结果完全一致。所描述的方法能够获得用于细胞学的有用诊断涂片。此外，CB尖端和用于运输的盐溶液接种均产生了准确的细菌学结果。
本研究中描述的使用CB进行细胞学和细菌学分析的方案可用于子宫内膜炎的诊断。为了使诊断样本质量最大化，必须在现场精心制备细胞学玻片，以保持细胞完整性并尽量减少人为假象。
