Laboratory of Phytopathology, Agricultural University of Athens, 75 Iera Odos str., 11855 Athens, Greece.
J Appl Microbiol. 2024 Apr 1;135(4). doi: 10.1093/jambio/lxae094.
This study explores the biocontrol potential of Pseudomonas putida Z13 against Botrytis cinerea in tomato plants, addressing challenges posed by the pathogen's fungicide resistance. The aims of the study were to investigate the in vitro and in silico biocontrol traits of Z13, identify its plant-colonizing efficacy, evaluate the efficacy of different application strategies against B. cinerea in planta, and assess the capacity of Z13 to trigger induced systemic resistance (ISR) in plants.
The in vitro experiments revealed that Z13 inhibits the growth of B. cinerea, produces siderophores, and exhibits swimming and swarming activity. Additionally, the Z13 genome harbors genes that encode compounds triggering ISR, such as pyoverdine and pyrroloquinoline quinone. The in planta experiments demonstrated Z13's efficacy in effectively colonizing the rhizosphere and leaves of tomato plants. Therefore, three application strategies of Z13 were evaluated against B. cinerea: root drenching, foliar spray, and the combination of root drenching and foliar spray. It was demonstrated that the most effective treatment of Z13 against B. cinerea was the combination of root drenching and foliar spray. Transcriptomic analysis showed that Z13 upregulates the expression of the plant defense-related genes PR1 and PIN2 upon B. cinerea inoculation.
The results of the study demonstrated that Z13 possesses significant biocontrol traits, such as the production of siderophores, resulting in significant plant protection against B. cinerea when applied as a single treatment to the rhizosphere or in combination with leaf spraying. Additionally, it was shown that Z13 root colonization primes plant defenses against the pathogen.
本研究探讨了假单胞菌 Z13 对番茄植株中灰葡萄孢的生物防治潜力，以应对该病原体对杀菌剂产生抗性的挑战。本研究的目的是研究 Z13 的体外和计算机模拟生物防治特性，确定其在植物上的定殖效果，评估不同应用策略对植物体内灰葡萄孢的防治效果，并评估 Z13 诱导植物系统抗性（ISR）的能力。
体外实验表明，Z13 抑制灰葡萄孢的生长，产生铁载体，并表现出游泳和群集活性。此外，Z13 基因组还包含编码触发 ISR 的化合物的基因，如绿脓菌素和吡咯喹啉醌。体内实验表明，Z13 能够有效地定殖番茄植株的根际和叶片。因此，评估了 Z13 对灰葡萄孢的三种应用策略：根部灌根、叶面喷雾和根部灌根与叶面喷雾相结合。结果表明，Z13 防治灰葡萄孢最有效的处理方法是根部灌根与叶面喷雾相结合。转录组分析表明，Z13 在接种灰葡萄孢后上调了与植物防御相关的基因 PR1 和 PIN2 的表达。
研究结果表明，Z13 具有显著的生物防治特性，如产生铁载体，单独应用于根际或与叶面喷雾相结合对灰葡萄孢具有显著的植物保护作用。此外，研究表明 Z13 的根定殖可使植物对病原体产生防御反应。
