Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia.
Group of Environment, Microbiology and Bioprocessing (GERMS), Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia.
Mol Biol Rep. 2024 Apr 11;51(1):503. doi: 10.1007/s11033-024-09492-8.
Komagataeibacter nataicola (K. nataicola) is a gram-negative acetic acid bacterium that produces natural bacterial cellulose (BC) as a fermentation product under acidic conditions. The goal of this work was to study the complete genome of K. nataicola and gain insight into the functional genes in K. nataicola that are responsible for BC synthesis in acidic environments.
The pure culture of K. nataicola was obtained from yeast-glucose-calcium carbonate (YGC) agar, followed by genomic DNA extraction, and subjected to whole genome sequencing on a Nanopore flongle flow cell. The genome of K. nataicola consists of a 3,767,936 bp chromosome with six contigs and 4,557 protein coding sequences. The maximum likelihood phylogenetic tree and average nucleotide identity analysis confirmed that the bacterial isolate was K. nataicola. The gene annotation via RAST server discovered the presence of cellulose synthase, along with three genes associated with lactate utilization and eight genes involved in lactate fermentation that could potentially contribute to the increase in acid concentration during BC synthesis.
A more comprehensive genome study of K. nataicola may shed light into biological pathway in BC productivity as well as benefit the analysis of metabolites generated and understanding of biological and chemical interactions in BC production later.
Komagataeibacter nataicola（K. nataicola）是一种革兰氏阴性醋酸菌，在酸性条件下作为发酵产物产生天然细菌纤维素（BC）。这项工作的目的是研究 K. nataicola 的完整基因组，并深入了解 K. nataicola 中负责在酸性环境中合成 BC 的功能基因。
从酵母-葡萄糖-碳酸钙（YGC）琼脂中获得 K. nataicola 的纯培养物，然后提取基因组 DNA，并在 Nanopore flongle 流池上进行全基因组测序。K. nataicola 的基因组由一个 3,767,936 bp 的染色体组成，有六个连续体和 4,557 个蛋白质编码序列。最大似然系统发育树和平均核苷酸同一性分析证实该细菌分离株为 K. nataicola。通过 RAST 服务器的基因注释发现了纤维素合酶的存在，以及与乳酸利用相关的三个基因和参与乳酸发酵的八个基因，这些基因可能有助于在 BC 合成过程中增加酸浓度。
对 K. nataicola 进行更全面的基因组研究，可以深入了解 BC 生产力的生物途径，并有助于分析生成的代谢物，以及理解 BC 生产中的生物和化学相互作用。
