Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia.
Center for Electromicrobiology, Department of Biology, Ny Munkegade 116, 8000 Aarhus C, Denmark.
Mar Pollut Bull. 2022 Sep;182:114022. doi: 10.1016/j.marpolbul.2022.114022. Epub 2022 Aug 10.
Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sediment pollution monitoring. The composition of the bacteria communities was determined using 16S rRNA V3-region gene amplicon sequencing, while hybrid whole-genome sequencing was employed to analyze the genome of Vibrio parahaemolyticus. The trace elements and antibiotic compositions were access using high-throughput spectrometry. The alpha- and beta-diversity of bacteria in gut and sediment samples from Kuantan differed substantially within (p-value = 0.017604) and between samples (p-value <0.007), respectively. Vibrio genera predominated in Kuantan samples, while Flavobacterium and Synechococcus_E genera predominated in Pekan samples. Vibrio parahaemolyticus revealed the presence of tet(35) and bla genes that conceived resistance towards tetracycline and beta-lactam antibiotics, respectively, which were detected in sediment and gut samples.
海洋环境中的抗生素污染对海洋生态系统构成了新的威胁。本研究旨在比较关丹和北根两个沿海地区的海参肠道和体腔微生物群与沉积物之间的差异，以期为沉积物污染监测提供潜在的生物标志物。细菌群落的组成通过 16S rRNA V3 区基因扩增子测序来确定，而混合全基因组测序则用于分析副溶血弧菌的基因组。痕量元素和抗生素组成则使用高通量光谱法进行分析。关丹肠道和沉积物样本的 alpha 和 beta 多样性在内部（p 值=0.017604）和样本之间有显著差异（p 值<0.007）。在关丹样本中，弧菌属占优势，而在北根样本中，黄杆菌属和聚球藻属占优势。副溶血弧菌显示存在 tet(35)和 bla 基因，分别对四环素和β-内酰胺类抗生素产生耐药性，这些基因在沉积物和肠道样本中均有检测到。
