一种从人诱导多能干细胞生成带附属器皮肤类器官的优化方案。
An optimized protocol for generating appendage-bearing skin organoids from human-induced pluripotent stem cells.
作者信息
Ahmed Imaan, Sun Jane, Brown Jason, Khosrotehrani Kiarash, Shafiee Abbas
机构信息
The University of Queensland Frazer Institute, The University of Queensland, Brisbane, QLD 4102, Australia.
Herston Biofabrication Institute, Metro North Hospital and Health Service, Brisbane, QLD 4029, Australia.
出版信息
Biol Methods Protoc. 2024 Mar 22;9(1):bpae019. doi: 10.1093/biomethods/bpae019. eCollection 2024.
Organoid generation from pluripotent stem cells is a cutting-edge technique that has created new possibilities for modelling human organs , as well as opening avenues for regenerative medicine. Here, we present a protocol for generating skin organoids (SKOs) from human-induced pluripotent stem cells (hiPSCs) via direct embryoid body formation. This method provides a consistent start point for hiPSC differentiation, resulting in SKOs with complex skin architecture and appendages (e.g. hair follicles, sebaceous glands, etc.) across hiPSC lines from two different somatic sources.
从多能干细胞生成类器官是一项前沿技术，为人类器官建模创造了新的可能性，也为再生医学开辟了道路。在此，我们展示了一种通过直接形成胚状体从人诱导多能干细胞（hiPSC）生成皮肤类器官（SKO）的方案。该方法为hiPSC分化提供了一个一致的起始点，可在来自两种不同体细胞来源的hiPSC系中产生具有复杂皮肤结构和附属器（如毛囊、皮脂腺等）的SKO。
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