Xu Minghao, Diao Lu, Xu Xiangxiang, Zhu Ao, Wang Yu, Liu Mi
Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, People's Republic of China.
Beijing Darwincell Biotech Co., Ltd., Building B1, Chang'An Miles of Shougang Park, Shijingshan District, Beijing 100043, People's Republic of China.
ACS Pharmacol Transl Sci. 2025 May 24;8(6):1768-1777. doi: 10.1021/acsptsci.5c00184. eCollection 2025 Jun 13.
Androgenetic alopecia (AGA) is the most prevalent type of hair loss in the clinic. Corticosteroids are the most widely used agents for hair loss treatment. However, the long-term use of hormonal drugs is associated with various side effects. Therefore, new treatment methods are urgently needed. In this study, we found that proteins derived from stressed mesenchymal stem cells (PDSSCs) can be utilized to treat AGA in a mouse model. Secretion from chemically stimulated mesenchymal stem cells (MSCs) can reverse hair loss through immune regulation and anti-inflammatory functions. Herein, the root extract of Atalantia monophylla is utilized to stimulate MSCs to produce stress proteins with immunomodulatory function, and thus, proteins derived from stressed MSCs (PDSSCs) can be obtained from stimulated MSCs. We found that PDSSCs successfully boosted hair growth and activated hair follicle (HF) stem cells, thereby inducing hair regeneration in an AGA mouse model. PDSSCs achieved this effect through the Wnt pathway and the TGF-β pathway. Our research is the first to illustrate that stressed-MSC therapy can improve hair regeneration in AGA mice. Overall, this study demonstrated that utilizing PDSSCs from MSCs stimulated with the root extract of Atalantia monophylla to treat AGA is a promising strategy.
雄激素性脱发（AGA）是临床上最常见的脱发类型。皮质类固醇是治疗脱发最广泛使用的药物。然而，长期使用激素类药物会带来各种副作用。因此，迫切需要新的治疗方法。在本研究中，我们发现应激间充质干细胞衍生蛋白（PDSSCs）可用于在小鼠模型中治疗AGA。化学刺激的间充质干细胞（MSCs）的分泌产物可通过免疫调节和抗炎功能逆转脱发。在此，单叶小芸木根提取物用于刺激MSCs产生具有免疫调节功能的应激蛋白，从而可从受刺激的MSCs中获得应激MSCs衍生蛋白（PDSSCs）。我们发现PDSSCs成功促进了毛发的生长并激活了毛囊（HF）干细胞，从而在AGA小鼠模型中诱导了毛发再生。PDSSCs通过Wnt通路和TGF-β通路实现了这一效果。我们的研究首次表明应激MSCs疗法可改善AGA小鼠的毛发再生。总体而言，本研究证明利用经单叶小芸木根提取物刺激的MSCs来源的PDSSCs治疗AGA是一种有前景的策略。
