Lee Soo Min, Lee Jin Ju, Kim Hun, Choi Gyung Ja
Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea.
Division of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Korea.
Plant Pathol J. 2024 Apr;40(2):151-159. doi: 10.5423/PPJ.OA.01.2024.0011. Epub 2024 Apr 1.
Bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) is one of the most severe diseases in radish cultivation. To control this plant disease, the most effective method has been known to cultivate resistant cultivars. Previously, we developed an efficient bioassay method for investigating resistance levels with 21 resistant and moderately resistant cultivars of radish against a strain Pcc KACC 10421. In this study, our research expanded to investigate the resistance of radish cultivars against six Pcc strains, KACC 10225, KACC 10421, ATCC 12312, ATCC 15713, LY34, and ECC 301365. To this end, the virulence of the six Pcc strains was determined based on the development of bacterial soft rot in seedlings of four susceptible radish cultivars. The results showed that the Pcc strains exhibited different virulence in the susceptible cultivars. To explore the race differentiation of Pcc strains corresponding to the resistance in radish cultivars, we investigated the occurrence of bacterial soft rot caused by the six Pcc strains on the 21 resistant and moderate resistant cultivars. Our results showed that the average values of the area under the disease progress curve were positively correlated with the virulence of the strains and the number of resistant cultivars decreased as the virulence of Pcc strains increased. Taken together, our results suggest that the resistance to Pcc of the radish cultivars commercialized in Korea is more likely affected by the virulence of Pcc strains rather than by race differentiation of Pcc.
胡萝卜软腐果胶杆菌胡萝卜软腐亚种（Pcc）引起的细菌性软腐病是萝卜种植中最严重的病害之一。为了防治这种植物病害，已知最有效的方法是培育抗性品种。此前，我们开发了一种高效的生物测定方法，用于研究21个萝卜抗性和中抗品种对Pcc菌株KACC 10421的抗性水平。在本研究中，我们的研究范围扩大到调查萝卜品种对6个Pcc菌株KACC 10225、KACC 10421、ATCC 12312、ATCC 15713、LY34和ECC 301365的抗性。为此，根据4个感病萝卜品种幼苗细菌性软腐病的发展情况，测定了6个Pcc菌株的毒力。结果表明，Pcc菌株在感病品种中表现出不同的毒力。为了探究与萝卜品种抗性相对应的Pcc菌株的生理小种分化，我们调查了6个Pcc菌株在21个抗性和中抗品种上引起的细菌性软腐病的发生情况。我们的结果表明，病情进展曲线下面积的平均值与菌株的毒力呈正相关，并且随着Pcc菌株毒力的增加，抗性品种的数量减少。综上所述，我们的结果表明，韩国商业化种植的萝卜品种对Pcc的抗性更可能受Pcc菌株毒力的影响，而不是受Pcc生理小种分化的影响。
