Rosenstein B S, Vaslet C A
Department of Radiation Oncology, Mount Sinai School of Medicine, City University of New York, NY 10029, USA.
J Photochem Photobiol B. 1995 Jun;28(3):203-11. doi: 10.1016/1011-1344(95)07115-i.
A mutant cell line, DRP 512, sensitive to the induction of non-dimer DNA damages produced by solar UV radiation was derived from ICR 2A frog cells. In addition, the DRP 512 cells exhibited an abnormally high level of ouabain-resistant mutants after exposure to solar UV. A level of 1.1. mutants per 10(6) survivors per kJ m-2 was measured for ICR 2A whereas the yield was 4.2 mutants per 10(6) survivors per kJ m-2 for the solar-UV-sensitive cell line. The DRP 512 cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity and mutation induction were restored. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and mutation induction to DRP 512. This gene has been designated SUVCC2.
