Rosenstein B S, Vaslet C A, Rosenstein R B
Department of Radiation Oncology, Mount Sinai School of Medicine, City University of New York, NY 10029.
Photochem Photobiol. 1995 Feb;61(2):142-8. doi: 10.1111/j.1751-1097.1995.tb03952.x.
A mutant cell line, DRP 287, sensitive to solar UV radiation and deficient in the repair of solar UV-induced nondimer DNA damage, was derived from ICR 2A frog cells. These cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity was restored and the repair defect corrected. The DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and correcting the repair defect in the DRP 287 cells. This represents the first human gene which has been isolated that is specifically involved in the repair of nondimer DNA damage induced by solar UV radiation. It has been designated SUVCC1 to denote solar UV cross-complementing gene number 1.
一种对太阳紫外线辐射敏感且在修复太阳紫外线诱导的非二聚体DNA损伤方面存在缺陷的突变细胞系DRP 287，是从ICR 2A蛙细胞中获得的。这些细胞用人类DNA进行转染，获得了一个二级转化体，其中恢复了正常的太阳紫外线敏感性并纠正了修复缺陷。来自该二级转化体的DNA被用于构建一个基因组DNA文库，从中分离出一个重组噬菌体，其包含能够恢复DRP 287细胞正常太阳紫外线敏感性并纠正修复缺陷的人类基因。这是分离出的第一个专门参与修复太阳紫外线辐射诱导的非二聚体DNA损伤的人类基因。它被命名为SUVCC1，以表示太阳紫外线交叉互补基因1。
