Su Xiaoyue, Zheng Junzan, Diao Xiaoxuan, Yang Zhongyi, Yu Deyue, Huang Fang
Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China.
Plants (Basel). 2024 Apr 2;13(7):1012. doi: 10.3390/plants13071012.
Plant structure has a large influence on crop yield formation, with branching and plant height being the important factors that make it up. We identified a gene, , encoding a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor highly conserved with gene (BRANCHED1) in . Sequence analysis revealed that included a conserved basic helix-loop-helix (BHLH) motif and R domain. Expression analysis showed that was expressed in all organs examined, with relatively higher expression in pods and axillary buds. Subcellular localization analysis showed that MtTCP18 was localized in the nucleus and exhibited transcriptional activation activity. These results supported its role as a transcription factor. Meanwhile, we identified a homozygous mutant line (NF14875) with a mutation caused by Tnt1 insertion into . Mutant analysis showed that the mutation of altered plant structure, with increased plant height and branch number. Moreover, we found that the expression of auxin early response genes was modulated in the mutant. Therefore, may be a promising candidate gene for breeders to optimize plant structure for crop improvement.
植物结构对作物产量形成有很大影响,分枝和株高是构成其的重要因素。我们鉴定出一个基因,编码一种与玉米中的TB1(BRANCHED1)基因高度保守的TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR(TCP)转录因子。序列分析表明,该基因包含一个保守的基本螺旋-环-螺旋(BHLH)基序和R结构域。表达分析表明,该基因在所有检测的器官中均有表达,在豆荚和腋芽中表达相对较高。亚细胞定位分析表明,MtTCP18定位于细胞核并表现出转录激活活性。这些结果支持了它作为转录因子的作用。同时,我们鉴定出一个纯合突变系(NF14875),其突变是由Tnt1插入该基因引起的。突变分析表明,该基因的突变改变了植物结构,增加了株高和分枝数。此外,我们发现突变体中生长素早期反应基因的表达受到调控。因此,该基因可能是育种者优化植物结构以改良作物的一个有前景的候选基因。