Mori Genki, Rahimian Sarira, Ozawa Rika, Murata Kenya, Hachisu Masakazu, Arimura Gen-Ichiro
Department of Biological Science and Technology, Faculty of Advanced Engineering, Tokyo University of Science, Tokyo 125-8585, Japan.
Center for Ecological Research, Kyoto University, Otsu 520-2113, Japan.
Plants (Basel). 2024 Apr 2;13(7):1015. doi: 10.3390/plants13071015.
Menthyl ester of valine (MV) has been developed as a plant defense potentiator to induce pest resistance in crops. In this study, we attempted to establish MV hydrochloride (MV-HCl) in lettuce and tomato crops. When MV-HCl solutions were used to treat soil or leaves of potted tomato and lettuce plants, 1 µM MV-HCl solution applied to potted plant soil was most effective in increasing the transcript level of defense genes such as (). As a result, leaf damage caused by and oviposition by were significantly reduced. In addition, MV-HCl-treated plants showed an increased ability to attract , a predatory mite of , when they were attacked by . Overall, our findings showed that MV-HCl is likely to be effective in promoting not only direct defense by activating defense genes, but also indirect defense mediated by herbivore-induced plant volatiles. Moreover, based on the results of the sustainability of expression in tomato plants treated with MV-HCl every 3 days, field trials were conducted and showed a 70% reduction in natural leaf damage. Our results suggest a practical approach to promoting organic tomato and lettuce production using this new plant defense potentiator.
缬氨酸薄荷酯（MV）已被开发为一种植物防御增强剂，用于诱导作物产生抗虫性。在本研究中，我们试图在生菜和番茄作物中应用盐酸缬氨酸薄荷酯（MV-HCl）。当用MV-HCl溶液处理盆栽番茄和生菜植株的土壤或叶片时，将1 µM的MV-HCl溶液施用于盆栽植物土壤中，对增加防御基因如（）的转录水平最为有效。结果，由（）造成的叶片损伤和（）的产卵量显著减少。此外，经MV-HCl处理的植株在受到（）攻击时，吸引（）（一种捕食螨）的能力增强。总体而言，我们的研究结果表明，MV-HCl不仅可能通过激活防御基因有效促进直接防御，还能促进由植食性昆虫诱导的植物挥发物介导的间接防御。此外，基于每3天用MV-HCl处理番茄植株后（）表达的持续性结果，进行了田间试验，结果显示自然叶片损伤减少了70%。我们的结果表明，使用这种新型植物防御增强剂是促进有机番茄和生菜生产的一种实用方法。
