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猪骨骼肌发育的分子调控：CDC23 表达与功能研究的启示

Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function.

机构信息

Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

TERRA Teaching and Research Center, University of Liège, Gembloux Agro-Bio Tech (ULiège-GxABT), 5030 Gembloux, Belgium.

出版信息

Int J Mol Sci. 2024 Mar 25;25(7):3664. doi: 10.3390/ijms25073664.

DOI:10.3390/ijms25073664
PMID:38612477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11011816/
Abstract

Cell division cycle 23 (CDC23) is a component of the tetratricopeptide repeat (TPR) subunit in the anaphase-promoting complex or cyclosome (APC/C) complex, which participates in the regulation of mitosis in eukaryotes. However, the regulatory model and mechanism by which the CDC23 gene regulates muscle production in pigs are largely unknown. In this study, we investigated the expression of CDC23 in pigs, and the results indicated that CDC23 is widely expressed in various tissues and organs. In vitro cell experiments have demonstrated that CDC23 promotes the proliferation of myoblasts, as well as significantly positively regulating the differentiation of skeletal muscle satellite cells. In addition, Gene Set Enrichment Analysis (GSEA) revealed a significant downregulation of the cell cycle pathway during the differentiation process of skeletal muscle satellite cells. The protein-protein interaction (PPI) network showed a high degree of interaction between genes related to the cell cycle pathway and CDC23. Subsequently, in differentiated myocytes induced after overexpression of CDC23, the level of CDC23 exhibited a significant negative correlation with the expression of key factors in the cell cycle pathway, suggesting that CDC23 may be involved in the inhibition of the cell cycle signaling pathway in order to promote the differentiation process. In summary, we preliminarily determined the function of CDC23 with the aim of providing new insights into molecular regulation during porcine skeletal muscle development.

摘要

细胞分裂周期蛋白 23（CDC23）是后期促进复合物或周期蛋白体（APC/C）复合物中四肽重复（TPR）亚基的组成部分，参与真核生物有丝分裂的调节。然而，CDC23 基因调节猪肌肉生成的调控模型和机制在很大程度上尚不清楚。在本研究中，我们研究了 CDC23 在猪中的表达，结果表明 CDC23 在各种组织和器官中广泛表达。体外细胞实验表明，CDC23 促进成肌细胞的增殖，并显著正向调节骨骼肌卫星细胞的分化。此外，基因集富集分析（GSEA）显示在骨骼肌卫星细胞分化过程中细胞周期途径显著下调。蛋白质-蛋白质相互作用（PPI）网络显示与细胞周期途径相关的基因与 CDC23 之间具有高度的相互作用。随后，在过表达 CDC23 诱导的分化肌细胞中，CDC23 的水平与细胞周期途径中的关键因子的表达呈显著负相关，表明 CDC23 可能参与抑制细胞周期信号通路以促进分化过程。总之，我们初步确定了 CDC23 的功能，旨在为猪骨骼肌发育过程中的分子调控提供新的见解。

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