Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China.
College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China.
Int J Mol Sci. 2024 Apr 7;25(7):4097. doi: 10.3390/ijms25074097.
Sunflower ( L.) is an important, substantial global oil crop with robust resilience to drought and salt stresses. The TGA (TGACG motif-binding factor) transcription factors, belonging to the basic region leucine zipper (bZIP) family, have been implicated in orchestrating multiple biological processes. Despite their functional significance, a comprehensive investigation of the TGA family's abiotic stress tolerance in sunflowers remains elusive. In the present study, we identified 14 TGA proteins in the sunflower genome, which were unequally distributed across 17 chromosomes. Employing phylogenetic analysis encompassing 149 TGA members among 13 distinct species, we revealed the evolutionary conservation of TGA proteins across the plant kingdom. Collinearity analysis suggested that both and were generated due to gene duplication. Notably, qRT-PCR analysis demonstrated that , , and genes were remarkably upregulated under ABA, MeJA, and salt treatments, whereas , , and were significantly repressed. This study contributes valuable perspectives on the potential roles of the gene family under various stress conditions in sunflowers, thereby enhancing our understanding of gene family dynamics and function within this agriculturally significant species.
向日葵（L.）是一种重要的、大量的全球油料作物，具有很强的抗旱和耐盐能力。TGA（TGACG 基序结合因子）转录因子属于碱性亮氨酸拉链（bZIP）家族，它们在协调多种生物学过程中起着重要的作用。尽管它们具有重要的功能，但对向日葵 TGA 家族在非生物胁迫耐受性方面的综合研究仍难以实现。在本研究中，我们在向日葵基因组中鉴定了 14 个 TGA 蛋白，它们在 17 条染色体上不均匀分布。通过对包括 13 个不同物种的 149 个 TGA 成员的系统发育分析，揭示了 TGA 蛋白在整个植物界的进化保守性。共线性分析表明， 和 是由于 基因复制产生的。值得注意的是，qRT-PCR 分析表明，ABA、MeJA 和盐处理下 、 、 和 基因显著上调，而 、 、 和 基因显著下调。本研究为在各种胁迫条件下 基因家族在向日葵中的潜在作用提供了有价值的见解，从而加深了我们对该农业重要物种中 基因家族动态和功能的理解。
