Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, 02-781 Warsaw, Poland.
Department of Biochemistry, Radioimmunology and Experimental Medicine, Children's Memorial Health Institute, 04-730 Warsaw, Poland.
Nutrients. 2024 Mar 22;16(7):917. doi: 10.3390/nu16070917.
The study investigated the impact of starch degradation products (SDexF) as prebiotics on obesity management in mice and overweight/obese children.
A total of 48 mice on a normal diet (ND) and 48 on a Western diet (WD) were divided into subgroups with or without 5% SDexF supplementation for 28 weeks. In a human study, 100 overweight/obese children were randomly assigned to prebiotic and control groups, consuming fruit and vegetable mousse with or without 10 g of SDexF for 24 weeks. Stool samples were analyzed for microbiota using 16S rRNA gene sequencing, and short-chain fatty acids (SCFA) and amino acids (AA) were assessed.
Results showed SDexF slowed weight gain in female mice on both diets but only temporarily in males. It altered bacterial diversity and specific taxa abundances in mouse feces. In humans, SDexF did not influence weight loss or gut microbiota composition, showing minimal changes in individual taxa. The anti-obesity effect observed in mice with WD-induced obesity was not replicated in children undergoing a weight-loss program.
SDexF exhibited sex-specific effects in mice but did not impact weight loss or microbiota composition in overweight/obese children.
本研究旨在探讨淀粉降解产物（SDexF）作为益生元对肥胖管理的影响，分别在肥胖症小鼠和超重/肥胖儿童中进行了研究。
将 48 只正常饮食（ND）的小鼠和 48 只西方饮食（WD）的小鼠分为补充或不补充 5%SDexF 的亚组，共进行 28 周的实验。在一项人体研究中，100 名超重/肥胖儿童被随机分为益生元组和对照组，食用含或不含 10 g SDexF 的果蔬慕斯 24 周。使用 16S rRNA 基因测序分析粪便样本中的微生物群，并评估短链脂肪酸（SCFA）和氨基酸（AA）。
结果表明，SDexF 可减缓两种饮食的雌性小鼠体重增加，但仅在雄性小鼠中为暂时缓解。它改变了小鼠粪便中的细菌多样性和特定类群的丰度。在人体中，SDexF 并未影响体重减轻或肠道微生物群组成，个体分类群的变化很小。在 WD 诱导肥胖的小鼠中观察到的抗肥胖作用在进行减肥计划的儿童中并未复制。
SDexF 在雄性小鼠中表现出性别特异性作用，但在超重/肥胖儿童中并未影响体重减轻或微生物群组成。
