编码人生长激素的DNA序列在大肠杆菌中的直接表达。
Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.
作者信息
Goeddel D V, Heyneker H L, Hozumi T, Arentzen R, Itakura K, Yansura D G, Ross M J, Miozzari G, Crea R, Seeburg P H
出版信息
Nature. 1979 Oct 18;281(5732):544-8. doi: 10.1038/281544a0.
DOI:10.1038/281544a0
PMID:386136
Abstract
DNA coding for human growth hormone was constructed by using chemically synthesised DNA in conjunction with enzymatically prepared cDNA. This 'hybrid' gene was expressed in Escherichia coli under the control of the lac promoter. A polypeptide was produced having the size and immunological properties characteristic of mature human growth hormone.
摘要
编码人生长激素的DNA是通过将化学合成的DNA与酶促制备的cDNA结合构建而成的。这个“杂交”基因在乳糖启动子的控制下在大肠杆菌中表达。产生了一种具有成熟人生长激素特征大小和免疫特性的多肽。
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