大鼠生长激素结构基因的核苷酸序列及其在细菌中的扩增

Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

作者信息

Seeburg P H, Shine J, Martial J A, Baxter J D, Goodman H M

出版信息

Nature. 1977 Dec 8;270(5637):486-94. doi: 10.1038/270486a0.

Abstract

The primary structure of DNA containing the sequence for rat pituitary growth hormone mRNA has been determined. DNA was obtained by reverse transcription of polyadenylated RNA from cultured pituitary cells and from recombinant bacterial plasmids. The amino acid sequences for rat growth hormone and its precursor form have been deduced from the determined nucleotide sequences.

摘要

已确定了含有大鼠垂体生长激素信使核糖核酸（mRNA）序列的脱氧核糖核酸（DNA）的一级结构。DNA是通过对来自培养的垂体细胞的多聚腺苷酸化RNA以及重组细菌质粒进行逆转录而获得的。大鼠生长激素及其前体形式的氨基酸序列已从所确定的核苷酸序列中推导出来。