College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Department of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
J Biomater Sci Polym Ed. 2024 Jul;35(10):1571-1583. doi: 10.1080/09205063.2024.2340819. Epub 2024 Apr 13.
Nanometer zinc oxide (ZnONPs) offers strong antibacterial, wound healing, hemostatic benefits, and UV protection. Additionally, poly(hexamethylene biguanide)hydrochloride (PHMB) is an environmentally friendly polymer with strong bactericidal properties. However, the synergistic effect of the combination of ZnONPs and PHMB has not been previously explored. The purpose of this study is to explore the synergies of ZnONPs and PHMB and the healing efficacy of ZnO NPs-PHMB-hydrogel on skin wounds in mice infected with . Therefore, the mice were subjected to skin trauma to create a wound model and were subsequently infected with , and then divided into various experimental groups. The repair effect was evaluated by assessing the healing rate, as well as measuring the levels of TNF-α, IL-2, EGF, and TGF-β1 contents in the tissue. On the 4th and 9th days post-modeling, the Z-P group exhibited notably higher healing rates compared to the control group. However, on the 15th day, both the Z-P and AC groups achieved healing rates exceeding 99%. ZnO NPs-PHMB-hydrogel promoted the formation of a fully restored epithelium, increased new hair follicles and sebaceous glands beneath the epidermis, and markedly reduced inflammatory cell infiltration, which was markedly distinct from the control group. On the 7th day, the Z-P group exhibited significantly higher levels of EGF and TGF-β1, along with a considerable reduction in the TNF-α levels as compared with the control group. These results affirmed that ZnO NPs-PHMB-hydrogel effectively inhibits infection and accelerates skin wound healing.
纳米氧化锌 (ZnONPs) 具有很强的抗菌、促进伤口愈合、止血和防晒功能。此外，盐酸聚六亚甲基双胍 (PHMB) 是一种具有很强杀菌性能的环保聚合物。然而，ZnONPs 和 PHMB 的组合协同作用尚未被探索过。本研究旨在探索 ZnONPs 和 PHMB 的协同作用，以及 ZnO NPs-PHMB-水凝胶对感染 的小鼠皮肤伤口的愈合效果。因此，将小鼠的皮肤造成创伤，以创建一个伤口模型，然后使其感染 ，并随后分为不同的实验组。通过评估愈合率，以及测量组织中 TNF-α、IL-2、EGF 和 TGF-β1 含量来评估修复效果。在建模后的第 4 天和第 9 天，Z-P 组的愈合率明显高于对照组。然而，在第 15 天，Z-P 和 AC 组的愈合率均超过 99%。ZnO NPs-PHMB-水凝胶促进了完全恢复的上皮形成，增加了表皮下的新毛囊和皮脂腺，并显著减少了炎症细胞浸润，与对照组明显不同。在第 7 天，Z-P 组的 EGF 和 TGF-β1 水平明显升高，TNF-α 水平与对照组相比也有显著降低。这些结果证实，ZnO NPs-PHMB-水凝胶能有效抑制 感染并加速皮肤伤口愈合。
