Lin Shiyang, Cui Zhongqi, Luo Qingqiong, Li Chen, Zhang Yue, Yang Fengjiao, Chen Yichuan, Xu Chuansheng, Gao Yan, Zhao Shasha, Sun Fenyong, Shen Dandan, Wu Qi, Shi Shuo
Department of Laboratory Medicine, School of Chemical Science and Engineering, Shanghai Tenth People's Hospital of Tongji University, Tongji University, Shanghai, 200092, P. R. China.
Department of Clinical Laboratory Medicine, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, Shanghai, 200443, P. R. China.
Adv Sci (Weinh). 2025 Mar;12(9):e2412226. doi: 10.1002/advs.202412226. Epub 2025 Jan 13.
The healing of bacterial biofilm-infected wounds is a complex process, and the construction of emerging therapeutic modalities that regulate the microenvironment to magnify therapeutic effects and reduce biotoxicity is still highly challenging. Herein, an engineered microneedle (MN) patch is reported to mediate the efficient delivery of black phosphorus nanosheets (BP NSs) and copper peroxide nanodots (CP NDs) for dual nanodynamic sterilization and methicillin-resistant staphylococcus aureus (MRSA)-infected wound healing. Results demonstrate that the system can eliminate biofilm, reduce cytotoxicity, promote angiogenesis and tissue regeneration by the multiple advantages of chemodynamic therapy (CDT), enhanced photodynamic therapy (PDT), and improved degradation process from BP NSs to phosphate for promoting cell proliferation. Notably, the balance between excellent photodynamic stability and rapid degradability of BP NSs is maintained, and the improved degradation mechanism of BP NSs is vividly elucidated by density functional theory (DFT)-based molecular dynamics (MD) calculations. Furthermore, the transcriptional changes of treated MRSA-infected skin are studied using RNA-seq technology to reveal the potential therapeutic mechanism. As envisaged, the proposed MN patch provides a safe, easy, also highly effective approach to achieve the temporal regulation of sterilization and tissue regeneration for bacterial biofilm-infected wounds.
细菌生物膜感染伤口的愈合是一个复杂的过程，构建能够调节微环境以放大治疗效果并降低生物毒性的新型治疗方式仍然极具挑战性。在此，报道了一种工程化微针（MN）贴片，用于介导黑磷纳米片（BP NSs）和过氧化铜纳米点（CP NDs）的高效递送，以实现双重纳米动力杀菌和耐甲氧西林金黄色葡萄球菌（MRSA）感染伤口的愈合。结果表明，该系统可通过化学动力疗法（CDT）、增强光动力疗法（PDT）的多重优势以及从BP NSs到磷酸盐的降解过程改善以促进细胞增殖，从而消除生物膜、降低细胞毒性、促进血管生成和组织再生。值得注意的是，BP NSs保持了优异的光动力稳定性和快速降解性之间的平衡，并且基于密度泛函理论（DFT）的分子动力学（MD）计算生动地阐明了BP NSs的降解机制。此外，使用RNA测序技术研究了经治疗的MRSA感染皮肤的转录变化，以揭示潜在的治疗机制。如预期的那样，所提出的MN贴片为实现细菌生物膜感染伤口的杀菌和组织再生的时间调节提供了一种安全、简便且高效的方法。
