Shipa Ahmed M E, Kahilo Khaled A, Elshazly Samir A, Taher Ehab S, Nasr Nasr E, Alotaibi Badriyah S, Almadaly Essam A, Assas Mona, Abdo Walied, Abouzed Tarek K, Salem Abdulati Elsanusi, Kirci Damla, El-Seedi Hesham R, Refaey Mohamed S, Rizk Nermin I, Shukry Mustafa, Dorghamm Doaa A
Department of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.
Department of Basic Medical and Dental Sciences, Faculty of Dentistry, Zarqa University, Zarqa 13110, Jordan.
Reprod Toxicol. 2024 Jun;126:108586. doi: 10.1016/j.reprotox.2024.108586. Epub 2024 Apr 16.
This study examined the protective effects of a Petroselinum crispum (P. crispum) methanolic extract on reproductive dysfunction induced by acrylamide in male rats. A total of 40 rats were divided into four groups (n=10). The control group received distilled water, the acrylamide group received 10 mg/kg of acrylamide, the P. crispum group received 100 mg/kg of P. crispum extract, and the combined group was pretreated with P. crispum for two weeks before co-administration of P. crispum and acrylamide. All administrations were administered orally using a gastric tube for eight weeks. Acrylamide decreased testosterone levels but did not affect levels of FSH or LH. It also increased testicular levels of (MDA) malondialdehyde and reduced activity of (SOD) superoxide dismutase and impairment of sperm parameters. Furthermore, the administration of acrylamide resulted in an elevation of tumor necrosis factor-alpha (TNF-α) levels and a reduction in the levels of steroidogenic acute regulatory protein (STAR) and cytochrome P450scc (P450scc). Acrylamide negatively affected the histopathological outcomes, Johnsen's score, the diameter of seminiferous tubules, and the thickness of the germinal epithelium. It also upregulated the expression of NF-ĸB P65 and downregulated the expression of kinesin motor protein. In contrast, treatment with P. crispum extract restored the levels of antioxidant enzymes, improved sperm parameters, and normalized the gene expression of TNF-α, IL-10, IL-6, iNOS, NF-ĸB, STAR, CYP17A1, 17β-HSD and P450scc. It also recovered testicular histological parameters and immunoexpression of NF-ĸB P65 and kinesin altered by acrylamide. P. crispum showed protective effects against acrylamide-induced reproductive toxicity by suppressing oxidative damage and inflammatory pathways.
本研究考察了皱叶欧芹甲醇提取物对丙烯酰胺诱导的雄性大鼠生殖功能障碍的保护作用。总共40只大鼠被分为四组（n = 10）。对照组给予蒸馏水，丙烯酰胺组给予10 mg/kg的丙烯酰胺，皱叶欧芹组给予100 mg/kg的皱叶欧芹提取物，联合组在同时给予皱叶欧芹和丙烯酰胺之前先用皱叶欧芹预处理两周。所有给药均通过胃管口服，持续八周。丙烯酰胺降低了睾酮水平，但不影响促卵泡生成素（FSH）或促黄体生成素（LH）水平。它还增加了睾丸中丙二醛（MDA）的水平，降低了超氧化物歧化酶（SOD）的活性，并损害了精子参数。此外，丙烯酰胺的给药导致肿瘤坏死因子-α（TNF-α）水平升高，类固醇生成急性调节蛋白（STAR）和细胞色素P450scc（P450scc）水平降低。丙烯酰胺对组织病理学结果、约翰森评分、曲细精管直径和生精上皮厚度产生负面影响。它还上调了核因子-κB P65的表达，下调了驱动蛋白的表达。相比之下，皱叶欧芹提取物治疗可恢复抗氧化酶水平，改善精子参数，并使TNF-α、白细胞介素-10（IL-10）、白细胞介素-6（IL-6）、诱导型一氧化氮合酶（iNOS）、核因子-κB、STAR、细胞色素P450 17α酶（CYP17A1）、17β-羟类固醇脱氢酶（17β-HSD）和P450scc的基因表达正常化。它还恢复了睾丸组织学参数以及由丙烯酰胺改变的核因子-κB P65和驱动蛋白的免疫表达。皱叶欧芹通过抑制氧化损伤和炎症途径对丙烯酰胺诱导的生殖毒性具有保护作用。
