Uslu Hamit, Atila Uslu Gözde, Çoban Taha Abdulkadir, Mendil Ali Sefa, Toraman Emine, Şahin Mahmut, Özkaraca Mustafa
Department of Physiology, Faculty of Medicine, Erzincan Binali Yıldırım University, Erzincan, Turkey.
Department of Biochemistry, Faculty of Medicine, Erzincan Binali Yıldırım University, Erzincan, Turkey.
J Mol Histol. 2025 May 26;56(3):171. doi: 10.1007/s10735-025-10456-w.
Acrylamide (ACR) is a popular substance to which our exposure increases with the changes in our lifestyle and brings with it various health problems. In order to determine appropriate therapeutics against ACR damage, it is important to investigate the multiple mechanisms that may be effective in its pathophysiology. This study investigated the effects of ACR exposure on ovarian, uterine, and testicular tissues by considering different pathophysiological pathways. Male-control (MC), male-acrylamide (MACR), female-control (FC), and female-acrylamide (FACR) groups were formed. ACR was administered at a dose of 60 mg/kg for 5 days. ACR exposure decreased CAT and TrxR-specific activities, GSH levels, and Bcl-2 expression, while significantly increasing MDA, IL-6, and NFĸB p65 levels, caspase 3, and Bax expression in ovarian, uterine, and testicular tissues. Based on these results, it was determined that acrylamide induced damage in ovarian, uterine and testicular tissues through various pathways such as oxidative stress, inflammation, and apoptosis. Consequently, when selecting a therapeutic target, the substance whose efficacy is being investigated should be effective in these pathways. Furthermore, this study is the first to demonstrate the occurrence of bladder retention in both sexes following acrylamide exposure and will be an important step for future research.
丙烯酰胺（ACR）是一种常见物质，随着生活方式的改变，我们对其接触增加，并带来各种健康问题。为了确定针对ACR损伤的合适治疗方法，研究其病理生理学中可能有效的多种机制很重要。本研究通过考虑不同的病理生理途径，研究了ACR暴露对卵巢、子宫和睾丸组织的影响。组建了雄性对照组（MC）、雄性丙烯酰胺组（MACR）、雌性对照组（FC）和雌性丙烯酰胺组（FACR）。以60mg/kg的剂量给予ACR，持续5天。ACR暴露降低了卵巢、子宫和睾丸组织中CAT和TrxR的特异性活性、GSH水平以及Bcl-2表达，同时显著增加了MDA、IL-6和NFĸB p65水平、caspase 3以及Bax表达。基于这些结果，确定丙烯酰胺通过氧化应激、炎症和凋亡等多种途径诱导卵巢、子宫和睾丸组织损伤。因此，在选择治疗靶点时，所研究疗效的物质应在这些途径中有效。此外，本研究首次证明了丙烯酰胺暴露后两性均出现膀胱潴留，这将是未来研究的重要一步。
