Biological Engineering Laboratory, Faculty of Science and Technology, Sultan Moulay Slimane University, Beni Mellal, Morocco; Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY, USA.
Biological Engineering Laboratory, Faculty of Science and Technology, Sultan Moulay Slimane University, Beni Mellal, Morocco.
Physiol Behav. 2024 Jun 1;280:114548. doi: 10.1016/j.physbeh.2024.114548. Epub 2024 Apr 12.
Corn and soybean oils are among the most frequently used vehicles for water-insoluble compounds in toxicological studies. These two vegetable oils are nutrients and may induce some biological effects on animals that might interfere with the experimental results. However, their chronic effects on a developing brain have not been reported. This study aims to evaluate the neurobehavioral and brain biochemical effects of both oils on male and female Swiss albino mice. Pregnant female mice were exposed to 1 µl/g/d of either tap water, corn oil (CO), or soybean oil (SO) from early gestation (GD1) until weaning then offspring mice were exposed to the same treatment regimen until adulthood (PND70). Our results showed that developmental exposure to both oils induced body weight changes in offspring mice. In addition, we detected some behavioral abnormalities where both oil-treated groups showed a significant decrease in locomotor activity and greater levels of anxiety behavior. Moreover, our results suggest that continuous exposure to these oils may alter motor coordination, spatial memory and induce depression-like behavior in adult mice. These alterations were accompanied by increased malondialdehyde, superoxide dismutase, and glutathione peroxidase activities in specific brain regions. Together, these data suggest that exposure to CO and SO as vehicles in developmental studies may interfere with the behavioral response and brain redox homeostasis in offspring mice.
玉米油和大豆油是毒理学研究中最常用来溶解水不溶性化合物的载体之一。这两种植物油是营养物质，可能会对动物产生一些生物学影响，从而干扰实验结果。然而，它们对发育中大脑的慢性影响尚未有报道。本研究旨在评估这两种油对雄性和雌性瑞士白化病小鼠的神经行为和大脑生化的影响。从妊娠早期（GD1）到断奶，怀孕的雌性小鼠接受 1µl/g/d 的自来水、玉米油（CO）或大豆油（SO）暴露，然后幼鼠接受相同的处理方案直至成年（PND70）。我们的结果表明，发育过程中暴露于这两种油会改变幼鼠的体重。此外，我们还检测到一些行为异常，其中油处理组的运动活性显著降低，焦虑行为更为严重。此外，我们的结果表明，持续暴露于这些油可能会改变成年小鼠的运动协调能力、空间记忆并引起类似抑郁的行为。这些变化伴随着特定脑区丙二醛、超氧化物歧化酶和谷胱甘肽过氧化物酶活性的增加。总之，这些数据表明，在发育研究中作为载体的 CO 和 SO 的暴露可能会干扰幼鼠的行为反应和大脑氧化还原稳态。
