Garvan-Weizmann Centre for Cellular Genomics, Garvan Institute for Medical Research, Darlinghurst, NSW, Australia.
Present address: Statewide Genomics at NSW Health Pathology, Sydney, NSW, Australia.
Genome Biol. 2024 Apr 15;25(1):94. doi: 10.1186/s13059-024-03224-8.
Recent innovations in single-cell RNA-sequencing (scRNA-seq) provide the technology to investigate biological questions at cellular resolution. Pooling cells from multiple individuals has become a common strategy, and droplets can subsequently be assigned to a specific individual by leveraging their inherent genetic differences. An implicit challenge with scRNA-seq is the occurrence of doublets-droplets containing two or more cells. We develop Demuxafy, a framework to enhance donor assignment and doublet removal through the consensus intersection of multiple demultiplexing and doublet detecting methods. Demuxafy significantly improves droplet assignment by separating singlets from doublets and classifying the correct individual.
单细胞 RNA 测序（scRNA-seq）的最新创新为以细胞分辨率研究生物学问题提供了技术支持。将来自多个个体的细胞混合已成为一种常见策略，并且可以通过利用其固有的遗传差异将液滴分配给特定的个体。scRNA-seq 面临的一个隐含挑战是双联体 - 包含两个或更多细胞的液滴。我们开发了 Demuxafy，这是一个通过多种去复用和双联体检测方法的共识交集来增强供体分配和去除双联体的框架。Demuxafy 通过将单体与双联体分离并对正确的个体进行分类，显著提高了液滴的分配。
