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联合治疗是否更佳：游泳运动和间歇性禁食通过miR-122-5p/SREBP-1c/CPT1A途径改善高脂饮食诱导的肥胖大鼠非酒精性脂肪性肝病

Would Combination Be Better: Swimming Exercise and Intermittent Fasting Improve High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease in Obese Rats via the miR-122-5p/SREBP-1c/CPT1A Pathway.

作者信息

Yang Kang, Liu Chengye, Shao Jun, Guo Lingxiang, Wang Qing, Meng Zhaoxiang, Jin Xing, Chen Xianghe

机构信息

Rehabilitation Medicine Department, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou city, Jiangsu Province, People's Republic of China.

Cardiovascular Disease Center, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou city, Jiangsu Province, People's Republic of China.

出版信息

Diabetes Metab Syndr Obes. 2024 Apr 12;17:1675-1686. doi: 10.2147/DMSO.S448165. eCollection 2024.

DOI:10.2147/DMSO.S448165
PMID:38623310
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11016699/
Abstract

BACKGROUND

Swimming and intermittent fasting can both improve obesity-induced NAFLD, but which of the two is more effective and whether the combination of the two has a superimposed effect is inconclusive.

METHODS

The model of NAFLD in obese rats was established by a high-fat diet and performed swimming, intermittent fasting, and a combination of both interventions for 8 weeks. Serum lipids and enzyme activity were measured by an automatic biochemical analyzer. Liver morphostructural analysis was observed by transmission electron microscopy, and morphology was observed by HE staining. RT‒PCR was used to detect the mRNA level.

RESULTS

Morphology and microstructure of the liver of model rats were impaired, with the upregulation of miR-122-5p, SREBP-1c, FASN and ACC1. Eight weeks of swimming exercise, intermittent fasting and the combination of both attenuate these effects, manifested by the downregulation of miR-122-5p and upregulation of CPT1A mRNA levels. There was no significant stacking effect of the combination of the swimming and intermittent fasting interventions.

CONCLUSION

NAFLD leads to pathology in model rats. Eight weeks of swimming exercise, intermittent fasting and the combination of both can inhibit miR-122-5p and improve hepatic lipid metabolism, while no significant additive effects of combining the interventions were found.

摘要

背景

游泳和间歇性禁食均可改善肥胖诱导的非酒精性脂肪性肝病（NAFLD），但二者哪种更有效以及二者联合是否具有叠加效应尚无定论。

方法

通过高脂饮食建立肥胖大鼠非酒精性脂肪性肝病模型，并进行游泳、间歇性禁食以及二者联合干预8周。采用自动生化分析仪检测血脂和酶活性。通过透射电子显微镜观察肝脏形态结构分析，采用苏木精-伊红（HE）染色观察形态学变化。运用逆转录-聚合酶链反应（RT-PCR）检测mRNA水平。

结果

模型大鼠肝脏的形态和微观结构受损，伴有微小RNA-122-5p（miR-122-5p）、固醇调节元件结合蛋白-1c（SREBP-1c）、脂肪酸合酶（FASN）和乙酰辅酶A羧化酶1（ACC1）上调。8周的游泳运动、间歇性禁食以及二者联合均减弱了这些影响，表现为miR-122-5p下调和肉碱棕榈酰转移酶1A（CPT1A）mRNA水平上调。游泳和间歇性禁食联合干预未产生显著的叠加效应。

结论

非酒精性脂肪性肝病导致模型大鼠出现病理变化。8周的游泳运动、间歇性禁食以及二者联合均可抑制miR-122-5p并改善肝脏脂质代谢，然而未发现联合干预有显著的相加效应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/92729d3e3d30/DMSO-17-1675-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/8c0a9b780e82/DMSO-17-1675-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/8c8b2998811b/DMSO-17-1675-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/0c03d2a04c28/DMSO-17-1675-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/d9dc0859f158/DMSO-17-1675-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/47ebd261aff2/DMSO-17-1675-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/92729d3e3d30/DMSO-17-1675-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/8c0a9b780e82/DMSO-17-1675-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/8c8b2998811b/DMSO-17-1675-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/0c03d2a04c28/DMSO-17-1675-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/d9dc0859f158/DMSO-17-1675-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/47ebd261aff2/DMSO-17-1675-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2929/11016699/92729d3e3d30/DMSO-17-1675-g0006.jpg

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