Research Center of Genetic Resources, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Institute for Plant Protection, NARO, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006326.
Phytopathogenic bacteria (MAFF 302110 and MAFF 302107) were isolated from lesions on Japanese angelica trees affected by bacterial soft rot in Yamanashi Prefecture, Japan. The strains were Gram-reaction-negative, facultatively anaerobic, motile with peritrichous flagella, rod-shaped, and non-spore-forming. The genomic DNA G+C content was 51.1 mol % and the predominant cellular fatty acids included summed feature 3 (C ω7 and/or C ω6), C, summed feature 8 (C ω7 and/or C ω6), summed feature 2 (comprising any combination of C aldehyde, an unknown fatty acid with an equivalent chain length of 10.928, C iso I, and C 3OH), and C. Phylogenetic analyses based on 16S rRNA and gene sequences, along with phylogenomic analysis utilizing whole-genome sequences, consistently placed these strains within the genus . However, their phylogenetic positions did not align with any known species within the genus. Comparative studies involving average nucleotide identity and digital DNA-DNA hybridization with the closely related species indicated values below the thresholds employed for the prokaryotic species delineation (95-96 % and 70 %, respectively), with the highest values observed for DPMP315 (92.10 and 47.1 %, respectively). Phenotypic characteristics, cellular fatty acid composition, and a repertoire of secretion systems could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic, and genotypic data obtained in this study show that MAFF 302110/MAFF 302107 represent a novel species of the genus , for which we propose the name sp. nov., designating MAFF 302110 (=ICMP 25161) as the type strain.
从日本山梨县受细菌性软腐病影响的当归植株病变部位分离到两种植物病原细菌（MAFF 302110 和 MAFF 302107）。这些菌株革兰氏反应阴性，兼性厌氧，周身鞭毛运动，杆状，不产芽孢。基因组 DNA G+C 含量为 51.1 mol %，主要细胞脂肪酸包括 sumFeature3（C ω7 和/或 C ω6）、C、sumFeature8（C ω7 和/或 C ω6）、sumFeature2（包含任何组合的 C 醛、同长 10.928 的未知脂肪酸、C iso I 和 C 3OH）和 C。基于 16S rRNA 和 基因序列的系统发育分析，以及利用全基因组序列进行的系统基因组分析，均将这些菌株置于 属内。然而，它们的系统发育位置与属内任何已知种均不吻合。涉及平均核苷酸同一性和与密切相关种的数字 DNA-DNA 杂交的比较研究表明，这些值低于用于原核种划分的阈值（分别为 95-96%和 70%），与 DPMP315 观察到的最高值分别为 92.10%和 47.1%。表型特征、细胞脂肪酸组成和一系列分泌系统可将这些菌株与它们最亲近的亲缘关系区分开来。本研究获得的表型、化学分类学和基因型数据表明，MAFF 302110/MAFF 302107 代表了 属的一个新种，我们将其命名为 sp. nov.，指定 MAFF 302110（=ICMP 25161）为模式株。
