Research Center of Genetic Resources, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Institute for Plant Protection, NARO, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
Int J Syst Evol Microbiol. 2022 Jun;72(6). doi: 10.1099/ijsem.0.005424.
Phytopathogenic bacterial strains (MAFF 311094, MAFF 311095, MAFF 311096 and MAFF 311097), which were isolated from rot lesions of parsley () sampled in Miyagi, Japan, were subjected to polyphasic characterization to determine their taxonomic position. The cells were Gram-reaction-negative, aerobic, non-spore-forming, motile with one or two polar flagella and rod-shaped. The 16S rRNA gene sequences analyses revealed that the strains belong to the genus , exhibiting the highest sequence similarity to P7 (99.93% sequence similarity), MAFF 301449 (99.93 %), 14-3 (99.86 %), MAFF 212408 (99.86 %) and CMS 35 (99.79 %). The genomic DNA G+C content was 60.1 mol%, and the major cellular fatty acids (>5 % of the total fatty acids) were C, summed feature 3 (C 7/C 6), summed feature 8 (C 7/C 6) and C cyclo. The sequence-based phylogenetic and whole genome-based phylogenomic analyses demonstrated that the strains are a member of the subgroup, but their phylogenetic position does not match those of any members of this subgroup. The average nucleotide identity and digital DNA-DNA hybridization values between the strains and their closely related species were ≤90.64% and ≤41.9 %, respectively, which were below the thresholds for prokaryotic species delineation (95-96 and 70%, respectively). Phenotypic characteristics, pathogenicity toward parsley and cellular fatty acid composition could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic and genotypic data presented in this study revealed that the strains constitute a novel species, for which we propose the name sp. nov., with MAFF 311094 (=ICMP 24279) being the type strain.
从日本宫城县采集的欧芹腐烂病斑中分离得到的植物病原细菌菌株（MAFF 311094、MAFF 311095、MAFF 311096 和 MAFF 311097），通过多相特征分析来确定其分类地位。这些细胞革兰氏反应阴性、需氧、不产芽孢、有一根或两根极生鞭毛，呈杆状。16S rRNA 基因序列分析表明，这些菌株属于欧文氏菌属，与 P7（99.93%序列相似性）、MAFF 301449（99.93%）、14-3（99.86%）、MAFF 212408（99.86%）和 CMS 35（99.79%）的相似度最高。基因组 DNA G+C 含量为 60.1mol%，主要细胞脂肪酸（占总脂肪酸的>5%）为 C、特征 3 总和（C 7/C 6）、特征 8 总和（C 7/C 6）和 C 环。基于序列的系统发育和全基因组系统发育基因组分析表明，这些菌株是 亚组的一个成员，但它们的系统发育位置与该亚组的任何成员都不匹配。这些菌株与其密切相关的种之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为≤90.64%和≤41.9%，均低于原核生物种划分的阈值（分别为 95-96%和 70%）。菌株与最接近的亲缘关系之间的表型特征、对欧芹的致病性和细胞脂肪酸组成可以将它们区分开来。本研究中的表型、化学分类和遗传数据表明，这些菌株构成了一个新的欧文氏菌属物种，我们建议将其命名为 sp. nov.，MAFF 311094（=ICMP 24279）为其模式株。
