Sawada Hiroyuki, Fujikawa Takashi, Osada Shigeru, Satou Mamoru
Genetic Resources Center, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Institute of Fruit Tree and Tea Science, NARO, 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605, Japan.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004723. Epub 2021 Mar 2.
Five phytopathogenic bacterial strains, MAFF 301449, MAFF 301450, MAFF 301451, MAFF 301452, and MAFF 301453, which were isolated from bud blight lesions of cyclamen ( Mill.) in Miyagi, Japan, were subjected to polyphasic taxonomic characterisation. The cells were Gram-reaction-negative, aerobic, non-spore-forming, motile with one to five polar flagella, and rod-shaped. Analysis of 16S rRNA gene sequences showed that they belong to the genus , with 14-3 (99.79 % sequence similarity), DSM 14937 (99.79 %), DSM 14936 (99.72 %), and CMS 35 (99.72 %) as their relatives. The genomic DNA G+C content was 60.3 mol% and the major fatty acids (>5 % of the total fatty acids) were C, summed feature 3 (C ω7/C ω6), summed feature 8 (C ω7/C ω6), and C cyclo. Phylogenetic analysis using the gene sequences and phylogenomic analyses based on the whole genome sequences demonstrated that the strains are members of the subgroup, but form a monophyletic and robust clade separated from their relatives. Average nucleotide identity and digital DNA-DNA hybridisation analyses with the closely related species corroborated their novel species status. The strains were differentiated from their relatives by phenotypic characteristics, pathogenicity towards cyclamen, cellular fatty acid composition, and whole-cell MALDI-TOF mass spectrometry profiles. Based on the phenotypic, chemotaxonomic, and genotypic data obtained, we conclude that the strains represent a novel species, for which we propose the name sp. nov.; the type strain is MAFF 301449 (=ICMP 23720).
从日本宫城县仙客来（仙客来属）芽枯病病斑中分离出的5株植物病原细菌菌株MAFF 301449、MAFF 301450、MAFF 301451、MAFF 301452和MAFF 301453，进行了多相分类学特征分析。这些细胞革兰氏染色阴性、需氧、不产芽孢、具1至5根极生鞭毛、杆状。16S rRNA基因序列分析表明，它们属于 属，与 14-3（序列相似性99.79%）、DSM 14937（99.79%）、DSM 14936（99.72%）和CMS 35（99.72%）亲缘关系较近。基因组DNA G+C含量为60.3 mol%，主要脂肪酸（占总脂肪酸的>5%）为C、总和特征3（C ω7/C ω6）、总和特征8（C ω7/C ω6）和C环。使用 基因序列进行的系统发育分析以及基于全基因组序列的系统基因组分析表明，这些菌株是 亚群的成员，但形成了一个与它们的亲缘种分离的单系且稳健的分支。与密切相关的 种进行的平均核苷酸同一性和数字DNA-DNA杂交分析证实了它们的新物种地位。这些菌株通过表型特征、对仙客来的致病性、细胞脂肪酸组成和全细胞MALDI-TOF质谱图谱与它们的亲缘种区分开来。基于获得的表型、化学分类学和基因型数据，我们得出结论，这些菌株代表一个新的 种，我们将其命名为 新种；模式菌株为MAFF 301449（=ICMP 23720）。
