Center for Rehabilitation Medicine, Department of Ophthalmology, Zhejiang Provincial People's Hospital (Affiliated People' Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, 310000, China.
He Eye Specialist Hospital, Shenyang, Liaoning Province, China.
BMC Ophthalmol. 2024 Apr 16;24(1):171. doi: 10.1186/s12886-024-03406-9.
To explore the safety of Neodymium:Yttrium-aluminum-garnet (Nd:YAG) laser vitreolysis based on the histological examination of the retina and the alteration of vitreous cytokines in the rabbits.
Nine male New Zealand rabbits underwent Nd:YAG laser vitreolysis of 10 mJ x 500 pulses in the left eyes, while the right eyes were used as controls. Intraocular pressure, color fundus photography, and ultrasound B scan were measured before, as well as 1 day, 4 weeks, and 12 weeks after Nd:YAG laser vitreolysis. Three rabbits were euthanized 1 day, 4 weeks, and 12 weeks after treatment, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and hematoxylin-eosin (H&E) staining were used to look for pathological changes in the retina. An enzyme-linked immunosorbent assay (ELISA) was utilized to detect the expression of vascular endothelial growth factor (VEGF) and some inflammatory cytokines, including interferon inducible protein 10 (IP-10), monocyte chemoattractant protein 1 (MCP-1) and interlenkin 6 (IL-6) in the vitreous humor. The ascorbic acid (AsA) and total reactive antioxidant potential (TRAP) in the vitreous humor were also measured.
Following Nd:YAG laser vitreolysis, the levels of VEGF, IP-10, MCP-1, IL6, AsA, and TRAP in the vitreous humor did not change substantially (P > 0.05). There were no detectable pathological changes in the retinal tissues, and no apoptotic signal was found.
Rabbits tolerate Nd:YAG laser vitreolysis without observable impact on retinal tissue or the microenvironment of the vitreous.
通过对新西兰兔视网膜组织学检查和玻璃体细胞因子改变，探讨钕：钇铝石榴石（Nd：YAG）激光玻璃体切割术的安全性。
9 只雄性新西兰兔左眼接受 10 mJ×500 脉冲 Nd：YAG 激光玻璃体切割术，右眼作为对照。在 Nd：YAG 激光玻璃体切割术前及术后 1 天、4 周和 12 周测量眼压、眼底彩色照相和超声 B 扫描。分别于治疗后 1 天、4 周和 12 周处死 3 只兔子。采用末端脱氧核苷酸转移酶 dUTP 缺口末端标记（TUNEL）染色和苏木精-伊红（H＆E）染色观察视网膜病理变化。采用酶联免疫吸附试验（ELISA）检测玻璃体血管内皮生长因子（VEGF）及干扰素诱导蛋白 10（IP-10）、单核细胞趋化蛋白 1（MCP-1）和白细胞介素 6（IL-6）等炎症细胞因子的表达。还测量了玻璃体中的抗坏血酸（AsA）和总反应性抗氧化能力（TRAP）。
Nd：YAG 激光玻璃体切割术后，玻璃体中 VEGF、IP-10、MCP-1、IL-6、AsA 和 TRAP 水平无明显变化（P>0.05）。视网膜组织无明显病理改变，未见凋亡信号。
新西兰兔能耐受 Nd：YAG 激光玻璃体切割术，对视网膜组织和玻璃体微环境无明显影响。
