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中国西南部壳斗科锥属1899年(法氏)的完整叶绿体基因组组装

The complete chloroplast genome assembly of Franch. 1899 (Fagaceae) in Southwestern China.

作者信息

Yin Si, Gao Yong

机构信息

College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan, China.

出版信息

Mitochondrial DNA B Resour. 2024 Apr 15;9(4):527-531. doi: 10.1080/23802359.2024.2342940. eCollection 2024.

Abstract

Franch. 1899 is one representative tree species distributed in the evergreen broad-leaved forests of southwestern China. This species is an important source of timber for manufacturing furniture, floors, and paper. It also plays a significant role in maintaining ecological balance and stability. To help with the genetic diversity assessment of , we sequenced and assembled the first complete chloroplast genome. The length of the chloroplast genome was 160,588 bp, with a typical quadripartite structure (GenBank accession no. OR900101). The large single-copy (LSC), small single-copy (SSC), and two inverted repeats (IRs) were 90,237 bp, 18,953 bp, and 25,699 bp in length, respectively. We annotated one hundred and thirty genes across the chloroplast genome, including 86 protein-coding genes (79 are unique), 37 tRNA genes (29 are unique), and eight rRNA genes (four are unique). Seventeen genes had one intron, and four were detected with two introns. The maximum likelihood phylogeny suggested that , , , and formed a clade with a high bootstrap value. This newly sequenced chloroplast genome assembly will aid in the population genetic and phylogenetic studies of species in the future.

摘要

栲树(Franch. 1899)是中国西南地区常绿阔叶林分布的一种代表性树种。该物种是制造家具、地板和纸张的重要木材来源。它在维持生态平衡和稳定方面也发挥着重要作用。为了帮助进行栲树的遗传多样性评估,我们对其首个完整叶绿体基因组进行了测序和组装。叶绿体基因组长度为160,588 bp,具有典型的四分体结构(GenBank登录号:OR900101)。大单拷贝区(LSC)、小单拷贝区(SSC)和两个反向重复序列(IRs)的长度分别为90,237 bp、18,953 bp和25,699 bp。我们对叶绿体基因组中的130个基因进行了注释,包括86个蛋白质编码基因(79个是独特的)、37个tRNA基因(29个是独特的)和8个rRNA基因(4个是独特的)。17个基因有一个内含子,4个基因有两个内含子。最大似然系统发育分析表明,栲树与其他相关物种形成了一个具有高自展值的分支。这个新测序的叶绿体基因组组装将有助于未来对栲树物种进行群体遗传学和系统发育研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb74/11020572/28b070a192fa/TMDN_A_2342940_F0001_C.jpg

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