Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil.
Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Laboratory of Emerging and Reemerging Pathogens, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil.
Future Microbiol. 2024;19(6):495-508. doi: 10.2217/fmb-2023-0208. Epub 2024 Apr 17.
To evaluate the action of promethazine, fluoxetine and carbonyl cyanide 3-chlorophenylhydrazone as efflux pump inhibitors (EPIs) against multidrug-resistant . The effect of the compounds was evaluated in planktonic cells and bacterial biofilms. Accumulation tests were performed with ethidium bromide to prove their action as EPIs. Then, they were associated with antimicrobials. Effect on planktonic cells and biofilms was found. Assays with ethidium bromide indicate their action as EPIs. Significant reductions in the metabolic activity of biofilms were observed after the association with the antimicrobials, especially for meropenem. It is possible to prove the action of these compounds as EPIs for and demonstrate the relevance of efflux pumps in antimicrobial resistance.
为评估普罗米嗪、氟西汀和羰基氰化物 3-氯苯腙作为外排泵抑制剂 (EPIs) 对多药耐药 的作用。评估了化合物在浮游细胞和细菌生物膜中的作用。用溴化乙锭进行积累试验以证明它们作为 EPIs 的作用。然后，它们与抗菌药物联合使用。发现对浮游细胞和生物膜有影响。溴化乙锭试验表明它们具有作为 EPIs 的作用。与抗菌药物联合使用后，生物膜的代谢活性明显降低，尤其是美罗培南。可以证明这些化合物对 具有 EPIs 作用，并证明外排泵在抗菌药物耐药性中的相关性。
