Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006339.
A novel Gram-stain-negative, yellow-pigmented, short rod-shaped bacterial strain, HBC34, was isolated from a freshwater sample collected from Daechung Reservoir, Republic of Korea. The results of 16S rRNA gene sequence analysis indicated that HBC34 was affiliated with the genus and shared the highest sequence similarity to the type strains of (98.01 %), (97.87 %) and (97.59 %). The average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH) values between HBC34 and species of the genus with validly published names were below 84.01 and 28.1 %, respectively. These values were lower than the accepted species-delineation thresholds, supporting its recognition as representing a novel species of the genus . The major fatty acids (>10 % of the total fatty acids) were identified as summed feature 8 (Cω7 and/or Cω6) and summed feature 3 (Cω7 and/or Cω6). The main polar lipids were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two phospholipids and two unidentified polar lipids. The respiratory quinone was Q-10. The genomic DNA G+C content of HBC34 was 64.04 %. The polyphasic evidence supports the classification of HBC34 as the type strain of a novel species of the genus for which the name sp. nov is proposed. The type strain is HBC34 (= KCTC 8002= LMG 33140).
一株新的革兰氏阴性、黄色、短杆状细菌菌株 HBC34，从韩国大邱水库的淡水样本中分离得到。16S rRNA 基因序列分析的结果表明，HBC34 属于 属，与模式菌株 （98.01%）、 （97.87%）和 （97.59%）的序列相似性最高。HBC34 与已发表的有效属名的种之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别低于 84.01%和 28.1%。这些值低于可接受的种区分阈值，支持将其视为代表 属的一个新种。主要脂肪酸（占总脂肪酸的>10%）被鉴定为特征 8（Cω7 和/或 Cω6）和特征 3（Cω7 和/或 Cω6）。主要极性脂为磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、两种磷脂和两种未鉴定的极性脂。呼吸醌为 Q-10。HBC34 的基因组 DNA G+C 含量为 64.04%。多相证据支持将 HBC34 分类为 属的一个新种的模式株，该新种命名为 sp. nov。模式株为 HBC34（=KCTC 8002=LMG 33140）。
