College of JunCao Science and Ecology (College of Carbon Neutrality), Fujian Agriculture and Forestry University, Fuzhou, China.
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.
Commun Biol. 2024 Oct 23;7(1):1377. doi: 10.1038/s42003-024-07085-6.
Small single-stranded DNA phages of the Microviridae family are diverse and prevalent in oceans. Our understanding of Microviridae phages that infect the ecologically important marine Roseobacter is currently limited, comprising few isolates. Here, we report six roseophages that infect Roseobacter RCA strains. Genomic and phylogenetic analyses revealed that they were new members of the previously identified subfamily Occultatumvirinae. Additionally, 232 marine uncultivated virus genomes (UViGs) affiliated to Occultatumvirinae were obtained from environmental genome datasets. Phylogenomic analysis revealed that marine Occultatumvirinae phages could be further grouped into 11 subgroups. Moreover, meta-omics based read-mapping analysis showed that Occultatumvirinae phages were globally distributed, with two low G + C subgroups showing the most prevalent distribution. Furthermore, one phage in subgroup 2 was found to be extremely ubiquitous. Overall, this study expands our understanding of the diversity and ecology of the Occultatumvirinae microviruses in the ocean and highlights their ecological impacts.
微病毒科的小型单链 DNA 噬菌体种类繁多，在海洋中广泛存在。我们对感染海洋玫瑰杆菌这一重要生态类群的微病毒的了解还很有限，目前仅分离到少数几种噬菌体。本研究报道了 6 种感染玫瑰杆菌 RCA 株的玫瑰噬菌体。基因组和系统发育分析表明，它们是先前确定的隐匿噬菌体亚科的新成员。此外，还从环境基因组数据集中获得了 232 种海洋未培养病毒基因组（UViGs），隶属于隐匿噬菌体亚科。系统发育分析表明，海洋隐匿噬菌体科的噬菌体可进一步分为 11 个亚群。此外，基于宏基因组的读映射分析表明，隐匿噬菌体科的噬菌体在全球范围内广泛分布，其中两个低 G+C 亚群的分布最为广泛。进一步研究发现，亚群 2 中的一种噬菌体分布极其普遍。总的来说，本研究扩展了我们对海洋中隐匿噬菌体科微病毒多样性和生态学的认识，并强调了它们的生态影响。
