Chen Yonghuan, Li Xiuying, Yue Xinru, Yu Weihua, Shi Yuesen, He Zilong, Wang Yuanfeng, Huang Yu, Xia Fan, Li Fengyu
College of Chemistry and Materials Science, Guangdong Provincial Key Laboratory of Speed Capability Research, Su Bingtian Center for Speed Research and Training, Jinan University, Guangzhou, PR China.
State Key Laboratory of Biogeology and Environmental Geology, Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan, PR China.
Nat Commun. 2025 Feb 21;16(1):1863. doi: 10.1038/s41467-025-57112-1.
Synthetic drugs fundamentally reshape the illicit drug market due to their low cost, ease of production, and rapid manufacturing processes. However, current drug detection methods, which rely on complex instruments, have limited applicability and often neglect the influence of pH fluctuations, leading to potential bias and unreliable results. Herein, we propose co-calibration DNA probes on a nanoconfined biosensor (NB), covering the range of sweat pH 3-8 to achieve significantly enhanced target signal recognition. The NB exhibits a linear response range of 10-10 fM with a low limit of detection (LOD) of 3.58 fM in artificial sweat. Compared to the single-aptamer NB, the dual-aptamer NB offers a broader linear response range, primarily due to the synergistic effects of changes in surface wettability and the capture of hydrion, which together reduce signal interference in proton transport. The linear response range doubles, and its detection sensitivity improves by 4-5 orders of magnitude compared to existing drug detection methods. This sensing strategy expands the application scope of aptamer-based composite probes, offering an approach for ultra-sensitive drug detection and demonstrating significant potential in sweat sensing and drug monitoring fields.
合成药物因其低成本、易于生产和快速制造工艺从根本上重塑了非法药物市场。然而，目前依赖复杂仪器的药物检测方法适用性有限，且常常忽视pH波动的影响，导致潜在的偏差和不可靠的结果。在此，我们提出在纳米受限生物传感器（NB）上进行共校准DNA探针，覆盖汗液pH 3 - 8的范围，以实现显著增强的目标信号识别。该NB在人工汗液中表现出10 - 10 fM的线性响应范围，检测限（LOD）低至3.58 fM。与单适配体NB相比，双适配体NB具有更宽的线性响应范围，这主要归因于表面润湿性变化和氢离子捕获的协同效应，它们共同减少了质子传输中的信号干扰。与现有药物检测方法相比，其线性响应范围翻倍，检测灵敏度提高了4 - 5个数量级。这种传感策略扩展了基于适配体的复合探针的应用范围，为超灵敏药物检测提供了一种方法，并在汗液传感和药物监测领域显示出巨大潜力。
