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用于基于运动性的微囊化益生菌功能检测的全光双模块平台。

All-optical dual module platform for motility-based functional scrutiny of microencapsulated probiotic bacteria.

作者信息

Wang Zhe, Giugliano Giusy, Behal Jaromir, Schiavo Michela, Memmolo Pasquale, Miccio Lisa, Grilli Simonetta, Nazzaro Filomena, Ferraro Pietro, Bianco Vittorio

机构信息

Institute of Applied Sciences and Intelligent Systems "E. Caianiello", National Research Council (ISASI-CNR), Via Campi Flegrei, 34, Pozzuoli, 80078, Italy.

Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, Piazzale Vincenzo Tecchio 80, Napoli 80125, Italy.

出版信息

Biomed Opt Express. 2024 Mar 6;15(4):2202-2223. doi: 10.1364/BOE.510543. eCollection 2024 Apr 1.

DOI:10.1364/BOE.510543
PMID:38633099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11019698/
Abstract

Probiotic bacteria are widely used in pharmaceutics to offer health benefits. Microencapsulation is used to deliver probiotics into the human body. Capsules in the stomach have to keep bacteria constrained until release occurs in the intestine. Once outside, bacteria must maintain enough motility to reach the intestine walls. Here, we develop a platform based on two label-free optical modules for rapidly screening and ranking probiotic candidates in the laboratory. Bio-speckle dynamics assay tests the microencapsulation effectiveness by simulating the gastrointestinal transit. Then, a digital holographic microscope 3D-tracks their motility profiles at a single element level to rank the strains.

摘要

益生菌被广泛应用于制药领域以提供健康益处。微囊化技术用于将益生菌递送至人体。胃中的胶囊必须在细菌释放至肠道之前将其限制住。一旦进入体外环境，细菌必须保持足够的运动能力以到达肠壁。在此，我们开发了一个基于两个无标记光学模块的平台，用于在实验室中快速筛选益生菌候选菌株并对其进行排名。生物散斑动力学分析通过模拟胃肠道转运来测试微囊化效果。然后，数字全息显微镜在单个细胞水平上对其运动特征进行三维跟踪，以对菌株进行排名。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/526e0ec2ba91/boe-15-4-2202-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/d0b7f978c117/boe-15-4-2202-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/21551205cc43/boe-15-4-2202-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/526e0ec2ba91/boe-15-4-2202-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/9024c8168604/boe-15-4-2202-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/3b5c8eeccbee/boe-15-4-2202-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/549b2e7a974f/boe-15-4-2202-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/34e028ca7de9/boe-15-4-2202-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/de7dcf68d2ec/boe-15-4-2202-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/d5af345fc983/boe-15-4-2202-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/82e6d30b75fd/boe-15-4-2202-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/8c46205d7f14/boe-15-4-2202-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/d0b7f978c117/boe-15-4-2202-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/21551205cc43/boe-15-4-2202-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af27/11019698/526e0ec2ba91/boe-15-4-2202-g011.jpg

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