Senftleben Maximilian Lukas, Bajor Antone, Hirata Eduardo, Abrahamsson Sara, Brismar Hjalmar
Department of Applied Physics, KTH Royal Institute of Technology, Science for Life Laboratory, Stockholm, Sweden.
Baskin School of Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, 95064, CA, USA.
Biomed Opt Express. 2024 Mar 11;15(4):2281-2292. doi: 10.1364/BOE.516261. eCollection 2024 Apr 1.
Studying the nanoscale dynamics of subcellular structures is possible with 2D structured illumination microscopy (SIM). The method allows for acquisition with improved resolution over typical widefield. For 3D samples, the acquisition speed is inherently limited by the need to acquire sequential two-dimensional planes to create a volume. Here, we present a development of multifocus SIM designed to provide high volumetric frame rate by using fast synchronized electro-optical components. We demonstrate the high volumetric imaging capacity of the microscope by recording the dynamics of microtubule and endoplasmatic reticulum in living cells at up to 2.3 super resolution volumes per second for a total volume of 30 × 30 × 1.8 µm.
使用二维结构照明显微镜（SIM）可以研究亚细胞结构的纳米级动力学。该方法能够以高于典型宽视场的分辨率进行采集。对于三维样本，由于需要采集连续的二维平面来构建一个体积，采集速度本质上受到限制。在此，我们展示了一种多焦点SIM的改进技术，该技术通过使用快速同步电光组件来提供高体积帧率。我们通过记录活细胞中微管和内质网的动力学，以每秒高达2.3个超分辨率体积的速度，对总体积为30×30×1.8 µm的样本进行成像，展示了该显微镜的高体积成像能力。
