Department of Food Control, Zagazig University, Zagazig, Egypt.
Open Vet J. 2024 Jan;14(1):176-185. doi: 10.5455/OVJ.2024.v14.i1.16. Epub 2024 Jan 31.
Food safety is a serious challenge in the face of increasing population and diminishing resources. is a critical foodborne pathogen characterized by its capability to secret a diverse range of heat-resistant enterotoxins. Antibiotic usage in dairy herds resulted in the occurrence of antimicrobial resistance (AMR) patterns among bacterial species, which were consequently transmitted to humans via dairy products. Lactic acid bacteria (LAB) produce bacteriocins, which provide an excellent source of natural antimicrobials with the further advantage of being environmentally friendly and safe.
Detection of multidrug resistance (MDR) isolates in concerned samples, molecular characteristics, biofilm production, and the inhibitory role of LAB against it.
Random samples of raw milk and other dairy products were analyzed for isolation. Phenotypic and genotypic assessment of AMR was performed, in addition to detection of classical enterotoxin genes of . Finally, evaluation of the antimicrobial action of some strains against .
Incidence rates of presumptive in raw milk, Kariesh cheese, and yogurt samples were 50%, 40%, and 60%, respectively. The highest resistance of was to Kanamycin (100%) and Nalidixic acid (89.3%), respectively. (78.66%) of were MDR. 11.1% of carried gene. In concern with enterotoxins genes, PCR showed that examined isolates harbored with a percentage of (22.2%), while was found in (11.1%) of isolates. Regarding biofilm production, (88.88%) of were biofilm producers. Finally, agar well diffusion showed that had the strongest antimicrobial action against with inhibition zone diameter ranging from 18 to 22 mm.
There is a widespread prevalence of MDR in raw milk and dairy products. Production of staphylococcal enterotoxins, as well as biofilm production are responsible for public health risks. Therefore, installing proper hygienic routines and harsh food safety policies at food chain levels is substantial.
食品安全是一个严峻的挑战，特别是在人口增长和资源减少的情况下。金黄色葡萄球菌是一种重要的食源性致病菌，其特点是能够分泌多种耐热肠毒素。在奶牛群中使用抗生素导致了细菌物种中出现抗生素耐药性（AMR）模式，这些模式随后通过乳制品传播给人类。乳酸菌（LAB）产生细菌素，这是一种极好的天然抗菌剂来源，具有环保和安全的进一步优势。
检测相关样本中的多药耐药（MDR）金黄色葡萄球菌分离株、分子特征、生物膜生成以及 LAB 对其的抑制作用。
对原料奶和其他乳制品的随机样本进行金黄色葡萄球菌分离。进行 AMR 的表型和基因型评估，此外还检测了金黄色葡萄球菌的经典肠毒素基因。最后，评估了一些 LAB 菌株对金黄色葡萄球菌的抗菌作用。
原料奶、卡雷什奶酪和酸奶样本中金黄色葡萄球菌的检出率分别为 50%、40%和 60%。金黄色葡萄球菌对卡那霉素（100%）和萘啶酸（89.3%）的耐药性最高。78.66%的金黄色葡萄球菌为多药耐药。11.1%的金黄色葡萄球菌携带肠毒素基因。关于肠毒素基因，PCR 显示，检测到的分离株携带基因的比例为 22.2%，而基因则存在于 11.1%的分离株中。关于生物膜生成，88.88%的金黄色葡萄球菌是生物膜生产者。最后，琼脂孔扩散法显示，LAB 对金黄色葡萄球菌的抗菌作用最强，抑菌圈直径为 18-22 毫米。
原料奶和乳制品中广泛存在多药耐药金黄色葡萄球菌。金黄色葡萄球菌肠毒素的产生以及生物膜的生成是对公众健康的威胁。因此，在食物链各级建立适当的卫生程序和严格的食品安全政策是至关重要的。
