Zhang Mian, Xiao Qiaoqiao, Li Yulong, Tian Yuan, Zheng Jincheng, Zhang Jie
Guizhou University of Traditional Chinese Medicine, Guiyang, 550025 China.
College of Life Sciences, Shaanxi Normal University, Xi'an, 710119 China.
Physiol Mol Biol Plants. 2024 Mar;30(3):453-466. doi: 10.1007/s12298-024-01435-8. Epub 2024 Mar 20.
In this study, we applied exogenous chlorogenic acid (CGA) to () leaves via foliar sprays every Monday, Wednesday, and Friday for a period of 12 months. Our continuous monitoring over this period revealed a consistent increase in flavonoid levels from the second to the tenth month following the commencement of CGA treatment. This was accompanied by a notable upregulation in the expression of four secondary metabolite-related enzyme genes: , , , and . Concurrently, there was a significant enhancement in the total activity of the enzyme phenylalanine ammonia-lyase. The total antioxidant capacity of the plants also showed a marked increase from the third to the seventh month post-treatment initiation, subsequently stabilizing. This increase was also reflected in the elevated activities of key antioxidant enzymes: peroxidase, polyphenol oxidase, and superoxide dismutase. Furthermore, the treatment notably enhanced various indicators of nutrient growth, such as total protein content, total sugar content, and leaf area. Notably, the relative expression of , a kind of BZIP transcription factor gene known for its extensive regulatory effects, showed a significant and sustained increase after the start of exogenous CGA treatment. Subsequent metabolomic analysis revealed significant changes in metabolites. Specifically, 172 differentially expressed metabolites (DEMs) showed a notable increase (Fold > 1), predominantly in pathways related to nutrient metabolism such as carbohydrate, amino acid, and energy metabolism. Notably, some of the highly expressed DEMs (Fold > 4) are key antioxidants and medicinal components in . The experimental findings were in alignment with the metabolomics analysis, indicating that exogenous CGA can act as a stimulant for . It promotes the significant accumulation of certain secondary metabolites, enhances nutritive growth, and boosts the plant's total antioxidant capacity.
The online version contains supplementary material available at 10.1007/s12298-024-01435-8.
在本研究中，我们于每周一、周三和周五通过叶面喷施的方式，对（）叶片施加外源绿原酸（CGA），持续12个月。在这段时间的持续监测显示，从CGA处理开始后的第二个月到第十个月，类黄酮水平持续上升。与此同时，四个与次生代谢物相关的酶基因：、、和的表达显著上调。同时，苯丙氨酸解氨酶的总活性显著增强。从处理开始后的第三个月到第七个月，植物的总抗氧化能力也显著增加，随后趋于稳定。这种增加也反映在关键抗氧化酶（过氧化物酶、多酚氧化酶和超氧化物歧化酶）活性的提高上。此外，该处理显著提高了各种营养生长指标，如总蛋白含量、总糖含量和叶面积。值得注意的是，一种以广泛调控作用著称的BZIP转录因子基因的相对表达在外源CGA处理开始后显著且持续增加。随后的代谢组学分析显示代谢物发生了显著变化。具体而言，172种差异表达代谢物（DEM）显著增加（倍数>1），主要集中在与营养代谢相关的途径，如碳水化合物、氨基酸和能量代谢。值得注意的是，一些高表达的DEM（倍数>4）是（）中的关键抗氧化剂和药用成分。实验结果与代谢组学分析一致，表明外源CGA可作为（）的刺激剂。它促进了某些次生代谢物的显著积累，增强了营养生长，并提高了植物的总抗氧化能力。
在线版本包含可在10.1007/s12298-024-01435-8获取的补充材料。
