Gülmez Abdurrahman, Emecen Ahmet Naci, Emek Mestan, Ünal Belgin, Ergünay Koray, Öktem İbrahim Mehmet Ali, Özbek Özgen Alpay
Medical Microbiology Laboratory, İstanbul Başakşehir Cam ve Sakura Hospital, İstanbul, Turkey.
Department of Public Health, Dokuz Eylül University School of Medicine, İzmir, Turkey.
Infect Dis Clin Microbiol. 2022 Jun 13;4(2):107-115. doi: 10.36519/idcm.2022.102. eCollection 2022 Jun.
West Nile Virus (WNV), which causes widespread outbreaks in different parts of the world, is a risk to public health in Turkey, too. Community-based study data are needed to identify measures against possible outbreaks. This study aimed to determine the seroprevalence of community-based WNV in Manisa and to investigate the relationship between sociodemographic and socioeconomic variables.
We included individuals older than two years of age (N=1,317,917) registered in the Manisa Province Family Medicine Information System. Selected participants (n=1233) were determined by a simple random sampling method. Specific IgG antibodies against WNV were investigated in serum samples using a commercial ELISA test (Euroimmun, Germany). The relationship between age, gender, location, education and income level, occupation, population density, altitude, the location of the toilet in the house, and the presence of hypertension, diabetes mellitus and cardiovascular disease variables were analyzed by chi-square, Fisher's exact test and t-test. Adjusted odds ratio (OR) with95% confidence interval (CI) for each variable were calculated by the logistic regression method to explain potential risks.
WNV IgG antibodies were detected in 47 (3.8%) sera samples by ELISA. Seroprevalence was significantly correlated with independent variables of advanced age, presence of hypertension, diabetes mellitus and cardiovascular disease, low level of education and income, living in low altitude areas and the location of the toilet. In multivariate analysis; age (every one-year increase) (OR:1.05; 95% CI:1.02-1.07; <0.001), equivalent annual income per capita below 3265 TL (OR:3.21; 95% CI: 1.53-6.73; =0.002), and living areas below 132 meters altitude (OR=3.21; 95% CI 1.26-8.15; =0.014) were found to be the risk factors for WNV seropositivity.
In Manisa province, WNV IgG seroprevalence was detected as 3.8% with ELISA method. Older age, low income and living in regions with a low altitude were found to be associated with increased seropositivity significantly.
西尼罗河病毒（WNV）在世界不同地区引发广泛疫情，对土耳其的公共卫生也构成威胁。需要基于社区的研究数据来确定应对可能疫情的措施。本研究旨在确定马尼萨地区基于社区的WNV血清阳性率，并调查社会人口统计学和社会经济变量之间的关系。
我们纳入了在马尼萨省家庭医学信息系统中登记的两岁以上个体（N = 1,317,917）。通过简单随机抽样方法确定选定参与者（n = 1233）。使用商业ELISA检测（德国欧蒙公司）在血清样本中检测针对WNV的特异性IgG抗体。通过卡方检验、Fisher精确检验和t检验分析年龄、性别、地点、教育程度和收入水平、职业、人口密度、海拔、房屋内厕所位置以及高血压、糖尿病和心血管疾病变量之间的关系。通过逻辑回归方法计算每个变量的调整优势比（OR）及其95%置信区间（CI），以解释潜在风险。
通过ELISA在47份（3.8%）血清样本中检测到WNV IgG抗体。血清阳性率与高龄、患有高血压、糖尿病和心血管疾病、教育程度和收入水平低、生活在低海拔地区以及厕所位置等独立变量显著相关。在多变量分析中；年龄（每增加一岁）（OR：1.05；95%CI：1.02 - 1.07；<0.001）、人均年等效收入低于3265土耳其里拉（OR：3.21；95%CI：1.53 - 6.73；=0.002）以及生活在海拔132米以下地区（OR = 3.21；95%CI 1.26 - 8.15；=0.014）被发现是WNV血清阳性的危险因素。
在马尼萨省，采用ELISA方法检测到WNV IgG血清阳性率为3.8%。发现高龄、低收入和生活在低海拔地区与血清阳性率显著增加有关。
