Karakoç Zehra Çağla, Tüzüner Bora Mete, Ergonul Onder, Pierro Anna, Di Fonzo Eugenio, Koruk İbrahim, Sambri Vittorio
1 Florence Nightingale Hospital , Infectious Diseases and Clinical Microbiology, Şişli, Turkey .
Vector Borne Zoonotic Dis. 2013 Oct;13(10):739-43. doi: 10.1089/vbz.2012.1140. Epub 2013 Jun 29.
We described the serological prevalence of West Nile Virus (WNV) antibodies among the human population in a historical and strategic region of Turkey. A serologic survey was conducted based on suspected cases in April, 2009, in the Mesopotamia region of Turkey, in the villages that were located alongside the Zergan River. All the sera were tested by enzyme-linked immunosorbent assay ELISA (Euroimmune™), and the positive samples were tested by immunofluorescent assay (IFA; Euroimmune™). As confirmation, neutralizing antibodies against WNV were tested by microneutralization assay (MNTA). In total, 307 individuals were included. The MNTA test was found to be positive among 52 individuals out of 307 (17%). In multivariate analysis, age >50 [odds ratio (OR)=5.2, confidence interval (CI) 2.76-9.97, p<0.001) and being in an occupational risk group (OR=2.02, CI 1.02-4.04, p=0.044) were found to be the risk factors for WNV seropositivity with the MNTA test. The physicians in the region should be aware of the risk of WNV infection and should be alerted to detect the clinical cases.
我们描述了土耳其一个具有历史意义和战略地位的地区人群中西尼罗河病毒（WNV）抗体的血清学流行情况。2009年4月，在土耳其美索不达米亚地区泽尔甘河沿岸的村庄，针对疑似病例开展了一项血清学调查。所有血清样本均采用酶联免疫吸附测定法（ELISA，Euroimmune™）进行检测，阳性样本再通过免疫荧光测定法（IFA；Euroimmune™）进行检测。作为确认手段，采用微量中和试验（MNTA）检测针对WNV的中和抗体。总共纳入了307名个体。在307名个体中，有52人（17%）的MNTA检测呈阳性。在多变量分析中，年龄>50岁[比值比（OR）=5.2，置信区间（CI）2.76 - 9.97，p<0.001]以及属于职业风险组（OR=2.02，CI 1.02 - 4.04，p=0.044）被发现是MNTA检测WNV血清阳性的风险因素。该地区的医生应意识到WNV感染的风险，并应提高警惕以发现临床病例。
