Kuloğlu Zeynep Ece, Talay Zeynep Gülçe, Albayrak Özgür, Ergönül Önder, Can Füsun
Koç University IsBank Research Center for Infectious Diseases (KUISCID), İstanbul, Türkiye.
Graduate School of Health Sciences, Koç University School of Medicine, İstanbul, Türkiye.
Infect Dis Clin Microbiol. 2023 Sep 30;5(3):257-261. doi: 10.36519/idcm.2023.246. eCollection 2023 Sep.
In this study, we aimed to investigate the changes in the B cell subpopulations after homologous or heterologous COVID-19 boosters. Blood samples were collected after baseline (3-5 months after two doses of CoronaVac), 1 and 3 months after BNT162b2 (n=28 and n=6), and CoronaVac (n=7 and n=4) boosters. Peripheral blood mononuclear cells (PBMCs) were isolated and stained with B cell markers, the ratios of naïve (CD19+CD20+CD27-), memory (CD19+CD20+CD27+), memory B cells expressing IgG (CD19+CD20+CD27+IgG+), and effector memory B cells (CD19+CD20+CD27+CD38+) were identified with flow cytometry. Significantly higher expression of memory B cells was observed in one month with BNT162b2 (12.16% one month, 5.98% three months) and CoronaVac (14.18% one month, 9.00% three months) boosters. IgG expressing memory B cell expression was significantly higher with BNT162b2 than with CoronaVac booster in one month (22.70% and 13.95%, respectively). The ratio of effector B cells in the first month after CoronaVac booster (25.44%) was significantly higher than the BNT162b2 booster (9.90%, =0.0263).
在本研究中，我们旨在调查同源或异源新冠病毒加强针接种后B细胞亚群的变化。在基线（两剂科兴疫苗接种后3 - 5个月）、BNT162b2加强针接种后1个月和3个月（n = 28和n = 6）以及科兴疫苗加强针接种后1个月和3个月（n = 7和n = 4）采集血样。分离外周血单个核细胞（PBMC）并用B细胞标志物染色，通过流式细胞术鉴定幼稚（CD19 + CD20 + CD27 -）、记忆（CD19 + CD20 + CD27 +）、表达IgG的记忆B细胞（CD19 + CD20 + CD27 + IgG +）和效应记忆B细胞（CD19 + CD20 + CD27 + CD38 +）的比例。在接种BNT162b2（1个月时为12.16%，3个月时为5.98%）和科兴疫苗（1个月时为14.18%，3个月时为9.00%）加强针后的1个月观察到记忆B细胞表达显著更高。接种BNT162b2加强针后1个月，表达IgG的记忆B细胞表达显著高于接种科兴疫苗加强针（分别为22.70%和13.95%）。接种科兴疫苗加强针后第1个月效应B细胞的比例（25.44%）显著高于接种BNT162b2加强针（9.90%，P = 0.0263）。
