Yang Yingchun, Yang Rong, Deng Fangqing, Yang Luqiu, Yang Guanghao, Liu Yanyan, Tian Qing, Wang Zixi, Li Aipeng, Shang Li, Cheng Genyang, Zhang Lianbing
Key Laboratory for Space Bioscience & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China.
School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
ACS Pharmacol Transl Sci. 2024 Mar 16;7(4):1055-1068. doi: 10.1021/acsptsci.3c00355. eCollection 2024 Apr 12.
An improved innate immunity will respond quickly to pathogens and initiate efficient adaptive immune responses. However, up to now, there have been limited clinical ways for effective and rapid consolidation of innate immunity. Here, we report that cutaneous irradiation with blue light of 450 nm rapidly stimulates the innate immunity through cell endogenous reactive oxygen species (ROS) regulation in a noninvasive way. The iron porphyrin-containing proteins, mitochondrial cytochrome c (Cyt-c), and cytochrome p450 (CYP450) can be mobilized by blue light, which boosts electron transport and ROS production in epidermal and dermal tissues. As a messenger of innate immune activation, the increased level of ROS activates the NF-κB signaling pathway and promotes the secretion of immunomodulatory cytokines in skin. Initiated from skin, a regulatory network composed of cytokines and immune cells is established through the circulation system for innate immune activation. The innate immunity activated by whole-body blue light irradiation inhibits tumor growth and metastasis by increasing the infiltration of antitumor neutrophils and tumor-associated macrophages. Our results elucidate the remote immune modulation mechanism of blue light and provide a clinically applicable way for innate immunity activation.
增强的先天免疫将对病原体迅速做出反应并启动有效的适应性免疫反应。然而，到目前为止，有效且快速巩固先天免疫的临床方法有限。在此，我们报告用450nm蓝光进行皮肤照射可通过以非侵入性方式调节细胞内源性活性氧（ROS）来快速刺激先天免疫。含铁血卟啉的蛋白质、线粒体细胞色素c（Cyt-c）和细胞色素P450（CYP450）可被蓝光调动，这增强了表皮和真皮组织中的电子传递和ROS生成。作为先天免疫激活的信使，ROS水平的升高激活NF-κB信号通路并促进皮肤中免疫调节细胞因子的分泌。从皮肤开始，通过循环系统建立由细胞因子和免疫细胞组成的调节网络以激活先天免疫。全身蓝光照射激活的先天免疫通过增加抗肿瘤中性粒细胞和肿瘤相关巨噬细胞的浸润来抑制肿瘤生长和转移。我们的结果阐明了蓝光的远程免疫调节机制，并为先天免疫激活提供了一种临床适用的方法。
