Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, ROC.
Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC.
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006322.
A novel mesophilic, hydrogenotrophic methanogen, strain CWC-04, was obtained from a sediment sample extracted from a gravity core retrieved at station 22 within the KP-9 area off the southwestern coast of Taiwan during the ORIII-1368 cruise in 2009. Cells of strain CWC-04 were rod-shaped, 1.4-2.9 µm long by 0.5-0.6 µm wide, and occurred singly. Strain CWC-04utilized formate, H/CO, 2-propanol/CO or 2-butanol/CO as catabolic substrates. The optimal growth conditions were 42 °C, 0.17 M NaCl and pH 5.35. The genomic DNA G+C content calculated from the genome sequence of strain CWC-04 was 46.19 mol%. Phylogenetic analysis of 16S rRNA gene revealed that strain CWC-04 is affiliated with the genus . The 16S rRNA gene sequences similarities within strains MRE50, SANAE and HZ254 were 93.7, 93.0 and 91.3 %, respectively. In addition, the optical density of CWC-04 culture dropped abruptly upon entering the late-log growth phase, with virus-like particles (150 nm in diameter) being observed on and around the cells. This observation suggests that strain CWC-04 harbours a lytic virus. Based on these phenotypic, phylogenetic and genomic results, we propose that strain CWC-04 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CWC-04 (=BCRC AR10050=NBRC 113165).
一株新型中温、产氢甲烷菌 CWC-04，是从 2009 年第三次海洋钻探计划（ORIII）1368 航次期间，在台湾西南海岸 KP-9 区站位 22 采集的重力柱状沉积物样本中分离得到的。CWC-04 菌株的细胞呈杆状，长 1.4-2.9μm，宽 0.5-0.6μm，单独存在。CWC-04 菌株利用甲酸盐、H/CO、2-丙醇/CO 或 2-丁醇/CO 作为分解代谢底物。最佳生长条件为 42°C、0.17 M NaCl 和 pH5.35。根据 CWC-04 菌株的基因组序列计算出的基因组 DNA G+C 含量为 46.19mol%。16S rRNA 基因的系统发育分析表明，CWC-04 菌株属于 属。MRE50、SANAE 和 HZ254 菌株的 16S rRNA 基因序列相似性分别为 93.7%、93.0%和 91.3%。此外，当 CWC-04 培养物进入对数后期生长阶段时，其培养液的吸光度会突然下降，并观察到细胞上和周围有类似病毒的颗粒（直径 150nm）。这一观察结果表明 CWC-04 菌株含有裂解性病毒。基于这些表型、系统发育和基因组结果，我们建议将 CWC-04 菌株代表一个新的属的新种，提议建立一个新的科，命名为 科，属名暂定为 gen. nov.，种名暂定为 sp. nov.。模式菌株为 CWC-04（=BCRC AR10050=NBRC 113165）。
