Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.
Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China.
Proteomics Clin Appl. 2024 Sep;18(5):e2300106. doi: 10.1002/prca.202300106. Epub 2024 Apr 19.
Cutibacterium acnes is a commensal bacterium residing in healthy skin and plays a critical role in maintaining skin homeostasis. C. acnes has been considered closely related to acne vulgaris, while recent studies suggest that C. acnes and its metabolites may have a protective role in atopic dermatitis (AD) by modulating the immune system and maintaining skin homeostasis. Extracellular vesicles (EVs) are small membranous vesicles secreted by bacteria that participate in bacteria-host interactions.
This study first compared C. acnes EVs from AD lesions (AD-EVs), acne lesions (Acne-EVs), and healthy skin (NC-EVs), using Label-free quantitative LC-MS/MS and validated differently expressed proteins by parallel reaction monitoring (PRM). Then Normal Human Epidermal Keratinocytes (NHEK) and human primary keratinocytes (KC) were treated with C. acnes EVs isolated from different groups, and the expressions of inflammatory factors were measured by quantitative real-time PCR and Western blotting.
Compared with the acne group, the AD group showed greater downregulation of proteins related to energy metabolism and carbon source utilization pathway. Differences in protein profile in AD and acne lesion-separated C. acnes EVs correspond to the abnormal sebum secretion pattern in both diseases. C. acnes EVs from different groups affected different expressions of Th1 and Th2 inflammatory factors and epidermal barrier markers in NHEK and KC, indicating different immunomodulatory potentials.
This study observed distinct proteomic differences between AD-EVs and Acne-EVs, and provided insights into the functional differences of C. acnes EVs in AD and acne.
痤疮丙酸杆菌是一种定植于健康皮肤的共生菌，在维持皮肤稳态中起着关键作用。痤疮丙酸杆菌被认为与寻常痤疮密切相关，而最近的研究表明，痤疮丙酸杆菌及其代谢产物可能通过调节免疫系统和维持皮肤稳态在特应性皮炎（AD）中发挥保护作用。细胞外囊泡（EVs）是细菌分泌的小膜性囊泡，参与细菌-宿主相互作用。
本研究首先使用无标记定量 LC-MS/MS 对 AD 病变（AD-EVs）、痤疮病变（Acne-EVs）和健康皮肤（NC-EVs）中的痤疮丙酸杆菌 EVs 进行比较，并通过平行反应监测（PRM）验证差异表达蛋白。然后用来自不同组的痤疮丙酸杆菌 EVs 处理正常人表皮角质形成细胞（NHEK）和人原代角质形成细胞（KC），通过定量实时 PCR 和 Western blot 测量炎症因子的表达。
与痤疮组相比，AD 组与能量代谢和碳源利用途径相关的蛋白表达下调更为明显。AD 和痤疮病变分离的痤疮丙酸杆菌 EVs 的蛋白图谱差异与两种疾病皮脂分泌模式异常相对应。来自不同组的痤疮丙酸杆菌 EVs 影响 NHEK 和 KC 中 Th1 和 Th2 炎症因子和表皮屏障标志物的不同表达，表明其具有不同的免疫调节潜力。
本研究观察到 AD-EVs 和 Acne-EVs 之间存在明显的蛋白质组差异，为痤疮丙酸杆菌 EVs 在 AD 和痤疮中的功能差异提供了新见解。
