Jarrousse V, Castex-Rizzi N, Khammari A, Charveron M, Dréno B
INSERM U601, 9 quai Moncousu, 44093 Nantes Cedex 01, France.
Arch Dermatol Res. 2007 Nov;299(9):441-7. doi: 10.1007/s00403-007-0774-5. Epub 2007 Aug 8.
Propionibacterium acnes plays an important role in the pathogenesis of acne and it is established that this bacteria is involved in the induction and maintenance of the inflammatory phase of acne. The aim of our work was to determine if P. acnes extracts could modulate integrins and filaggrin in vitro expression by keratinocytes. Integrins and filaggrin expression was examined using immunohistochemistry technique both on Normal Human Epiderminal Keratinocytes (NHEK) and on deep-frozen sections of normal human skin explants incubated with three different P. acnes extracts. In addition, the expression of filaggrin was investigated on biopsies of acne lesions and by western-blot associated with its precursor profilaggrin. We demonstrated that P. acnes extracts induced beta1 integrin expression significantly on both proliferating keratinocytes and differentiated keratinocytes. In addition, P. acnes induced alpha3, alpha6s and alphaVbeta6 integrin expression and filaggrin expression on differentiated keratinocytes. Finally P. acnes extracts increased filaggrin expression by suprabasal layer of epidermis of explants. Western-blot confirmed that total amount of filaggrin was increased. These results indicate that P. acnes extracts are directly able to modulate the differentiation of keratinocytes suggesting that this bacteria play a role not only in the development of inflammatory acne lesions but also in the formation of the microcomedo.
痤疮丙酸杆菌在痤疮发病机制中起重要作用，且已证实该细菌参与痤疮炎症期的诱导和维持。我们研究的目的是确定痤疮丙酸杆菌提取物是否能在体外调节角质形成细胞中整合素和丝聚合蛋白的表达。我们使用免疫组织化学技术，在正常人表皮角质形成细胞（NHEK）以及用三种不同痤疮丙酸杆菌提取物孵育的正常人皮肤外植体的冷冻切片上，检测整合素和丝聚合蛋白的表达。此外，我们还在痤疮皮损活检组织上以及通过与丝聚合蛋白前体前丝聚合蛋白相关的蛋白质印迹法，研究丝聚合蛋白的表达。我们证明，痤疮丙酸杆菌提取物在增殖的角质形成细胞和分化的角质形成细胞上均能显著诱导β1整合素表达。此外，痤疮丙酸杆菌在分化的角质形成细胞上诱导α3、α6s和αVβ6整合素表达以及丝聚合蛋白表达。最后，痤疮丙酸杆菌提取物使外植体表皮基底层以上的丝聚合蛋白表达增加。蛋白质印迹法证实丝聚合蛋白总量增加。这些结果表明，痤疮丙酸杆菌提取物能够直接调节角质形成细胞的分化，提示该细菌不仅在炎性痤疮皮损的发生中起作用，而且在微粉刺的形成中也起作用。
