College of Material, Chemistry, and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou 311121, China.
Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang 310036, China.
Sci Adv. 2024 Apr 19;10(16):eadl0263. doi: 10.1126/sciadv.adl0263.
The in situ generation of HO in cells in response to external stimulation has exceptional advantages in modulating intracellular Ca dynamics, including high controllability and biological safety, but has been rarely explored. Here, we develop photocatalyst-based metal-organic frameworks (DCSA-MOFs) to modulate Ca responses in cells, multicellular spheroids, and organs. By virtue of the efficient photocatalytic oxygen reduction to HO without sacrificial agents, photoexcited DCSA-MOFs can rapidly trigger Ca outflow from the endoplasmic reticulum with single-cell precision in a repeatable and controllable manner, enabling the propagation of intercellular Ca waves (ICW) over long distances in two-dimensional and three-dimensional cell cultures. After photoexcitation, ICWs induced by DCSA-MOFs can activate neural activities in the optical tectum of tadpoles and thighs of spinal frogs, eliciting the corresponding motor behaviors. Our study offers a versatile optical nongenetic modulation technique that enables remote, repeatable, and controlled manipulation of cellular and animal behaviors.
细胞内原位生成 HO 以响应外部刺激在调节细胞内 Ca 动力学方面具有独特的优势，包括高可控性和生物安全性，但尚未得到广泛探索。在这里，我们开发了基于光催化剂的金属有机骨架（DCSA-MOFs）来调节细胞、多细胞球体和器官中的 Ca 反应。凭借高效的无牺牲剂光催化氧气还原为 HO，光激发的 DCSA-MOFs 可以以单细胞精度、可重复且可控的方式迅速引发内质网中 Ca 的外流，从而在二维和三维细胞培养物中长距离传播细胞间 Ca 波（ICW）。光激发后，DCSA-MOFs 诱导的 ICWs 可激活蝌蚪光学顶盖和脊髓蛙大腿中的神经活动，引发相应的运动行为。我们的研究提供了一种通用的光学非遗传调制技术，可实现对细胞和动物行为的远程、可重复和可控操作。
