Department of Veterinary Physiology, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Mumbai, India.
Teaching Veterinary Clinical Complex, Mumbai Veterinary College, Maharashtra Animal and Fishery Sciences University, Mumbia, India.
BMC Vet Res. 2024 Apr 20;20(1):150. doi: 10.1186/s12917-024-04002-1.
Buffaloes have the highest potential for production due to a promising gene pool that is being enhanced and upgraded. Mastitis is a significant health impediment that greatly diminishes milk yield and quality, affecting rural farmers' livelihoods. The traditional gold standard used for diagnosing mastitis or subclinical mastitis is CMT, but it has the drawback of false positive or negative results. Subclinical mastitis, if not treated promptly, can lead to mammary tumors. To address the gap in early diagnosis of subclinical mastitis in CMT-negative milk of buffaloes, we performed a retrospective analysis and evaluated the milk miRNA expression profiles as potential biomarkers.
Thirty buffalo milk samples based on clinical signs and CMT were divided into normal, subclinical, and clinical mastitis. SCC evaluation showed significant differences between the groups. The data analysis demonstrated that the elevation of miR-146a and miR-383 differed substantially between normal, subclinical, and clinical mastitis milk of buffaloes with 100% sensitivity and specificity. The relationship of SCC with miR-146a and miR-383 in normal/healthy and subclinical mastitis was positively correlated.
The overexpression of miR-146a and miR-383 is associated with inflammation. It can be a valuable prognostic and most sensitive biomarker for early mastitis detection in buffaloes with SCC below 2 lakhs and CMT-ve, enhancing the accuracy of subclinical mastitis diagnosis.
水牛具有最高的生产潜力，因为其具有有前途的基因库，正在得到增强和升级。乳腺炎是一个严重的健康障碍，大大降低了牛奶产量和质量，影响了农村农民的生计。传统的金标准用于诊断乳腺炎或亚临床乳腺炎是 CMT，但它有假阳性或假阴性结果的缺点。如果不及时治疗亚临床乳腺炎，可能会导致乳腺肿瘤。为了解决 CMT 阴性水牛乳中亚临床乳腺炎早期诊断的空白，我们进行了回顾性分析，并评估了牛奶 miRNA 表达谱作为潜在的生物标志物。
根据临床症状和 CMT 将 30 份水牛牛奶样本分为正常、亚临床和临床乳腺炎。SCC 评估显示各组之间存在显著差异。数据分析表明，miR-146a 和 miR-383 的升高在正常/健康和亚临床乳腺炎水牛乳之间存在显著差异，具有 100%的灵敏度和特异性。SCC 与正常/健康和亚临床乳腺炎中 miR-146a 和 miR-383 的关系呈正相关。
miR-146a 和 miR-383 的过表达与炎症有关。对于 SCC 低于 2 lakhs 和 CMT-ve 的水牛，它可以作为一种有价值的预后和最敏感的生物标志物，用于早期乳腺炎检测，提高亚临床乳腺炎诊断的准确性。
