母体因子 Trim75 有助于小鼠早期胚胎的合子基因组激活程序。
Maternal factor Trim75 contributes to zygotic genome activation program in mouse early embryos.
机构信息
School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
College of Life Science, Northeast Forestry University, No. 26, hexing Road, Harbin, China.
出版信息
Mol Biol Rep. 2024 Apr 20;51(1):560. doi: 10.1007/s11033-024-09349-0.
BACKGROUND
Zygotic genome activation (ZGA) is an important event in the early embryo development, and human embryo developmental arrest has been highly correlated with ZGA failure in clinical studies. Although a few studies have linked maternal factors to mammalian ZGA, more studies are needed to fully elucidate the maternal factors that are involved in ZGA.
METHODS AND RESULTS
In this study, we utilized published single-cell RNA sequencing data from a Dux-mediated mouse embryonic stem cell to induce a 2-cell-like transition state and selected potential drivers for the transition according to an RNA velocity analysis.
CONCLUSIONS
An overlap of potential candidate markers of 2-cell-like-cells identified in this research with markers generated by various data sets suggests that Trim75 is a potential driver of minor ZGA and may recruit EP300 and establish H3K27ac in the gene body of minor ZGA genes, thereby contributing to mammalian preimplantation embryo development.
背景
合子基因组激活（ZGA）是早期胚胎发育中的一个重要事件，临床研究表明，人类胚胎发育停滞与 ZGA 失败高度相关。虽然有一些研究将母体因素与哺乳动物的 ZGA 联系起来，但仍需要更多的研究来充分阐明参与 ZGA 的母体因素。
方法和结果
在这项研究中，我们利用 Dux 介导的小鼠胚胎干细胞的已发表单细胞 RNA 测序数据，诱导出 2 细胞样过渡状态，并根据 RNA 速度分析选择过渡的潜在驱动因素。
结论
本研究中鉴定的 2 细胞样细胞的潜在候选标记与各种数据集生成的标记之间存在重叠，这表明 Trim75 是次要 ZGA 的潜在驱动因素，可能募集 EP300 并在次要 ZGA 基因的基因体中建立 H3K27ac，从而有助于哺乳动物着床前胚胎发育。
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