Noroozi Fatemeh, Asle-Rousta Masoumeh, Amini Rahim, Sahraeian Zeinab
Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Department of Biology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Iran J Basic Med Sci. 2024;27(6):678-684. doi: 10.22038/IJBMS.2024.73116.15890.
Renal and testicular disorders are primarily associated with oxidative damage and inflammation. Here, alpha-pinene (a type of monoterpene) was investigated for its effect on oxidative/nitrosative stress and the expression of inflammatory and apoptotic factors in the kidneys and testes of rats treated with CCl.
CCl was injected intraperitoneally (IP) at a dose of 2 ml/kg (twice a week for six weeks). Alpha-pinene (50 mg/kg/day, IP) was also treated during the same period.
CCl increased the level of malondialdehyde (<0.01 in the kidney and <0.001 in the testis) and nitric oxide (<0.001 in the kidney and <0.01 in the testis) and decreased the levels of glutathione (<0.05) in the kidneys and testicles of rats. CCl also reduced the catalase enzyme activity in the kidneys (<0.05) but did not affect its activity in the testis. In addition, CCl enhanced the mRNA expression of TNF-α (<0.01), nuclear factor-κB (P<0.05), and Bax (<0.05 in the kidney and <0.01 in the testis) and decreased the expression of Bcl-2 (<0.05) in both organs. Alpha-pinene prevented all the mentioned changes, but it did not influence the expression of Bcl-2 in the kidneys of rats receiving CCl.
Alpha-pinene may have the potential to prevent renal and testicular diseases by strengthening the antioxidant system in the kidneys and testis, and inhibiting oxidative/nitrosative stress, inflammation, and apoptosis caused by CCl.
肾脏和睾丸疾病主要与氧化损伤和炎症相关。在此，研究了α-蒎烯（一种单萜类化合物）对四氯化碳处理的大鼠肾脏和睾丸中氧化/亚硝化应激以及炎症和凋亡因子表达的影响。
以2 ml/kg的剂量腹腔注射四氯化碳（每周两次，共六周）。在此期间也对大鼠腹腔注射α-蒎烯（50 mg/kg/天）。
四氯化碳增加了丙二醛水平（肾脏中<0.01，睾丸中<0.001）和一氧化氮水平（肾脏中<0.001，睾丸中<0.01），并降低了大鼠肾脏和睾丸中谷胱甘肽的水平（<0.05）。四氯化碳还降低了肾脏中过氧化氢酶的活性（<0.05），但不影响其在睾丸中的活性。此外，四氯化碳增强了肿瘤坏死因子-α（TNF-α）（<0.01）、核因子-κB（P<0.05）和Bax（肾脏中<0.05，睾丸中<0.01）的mRNA表达，并降低了两个器官中Bcl-2的表达（<0.05）。α-蒎烯阻止了所有上述变化，但对接受四氯化碳处理的大鼠肾脏中Bcl-2的表达没有影响。
α-蒎烯可能具有通过增强肾脏和睾丸中的抗氧化系统，抑制四氯化碳引起的氧化/亚硝化应激、炎症和凋亡来预防肾脏和睾丸疾病的潜力。
